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US 12,203,935 B2Granted
Probe for universal detection of circulating tumor cells
AssigneeUniversity of Houston System
InventorsShaun Xiaoliu Zhang, Xinping Fu
Life of this patent
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Abstract
Disclosed are probes based on papilloma virus and modified SV40 that can be used for detecting circulating tumor cells (CTCs) in the blood stream, methods for manufacturing such probes, and methods for using such probes.
Claims14
A probe for detecting circulating tumor cells (CTCs), comprising:
- a modified SV40 viral genome packaged into a capsid formed from the L1 and L2 capsid proteins of a human, bovine, or other papillomavirus, and including the luciferase (luc) marker gene of SEQ ID NO:
- 10, wherein the modified SV40 viral genome also comprises an SV40 virus late promoter.
A process for assembling a probe that can detect circulating tumor cells (CTCs), the process comprising:
- co-transfecting papilloma virus L1 and L2 genes with a modified SV40 viral genome construct into mammalian cells, or by in vitro assembly of the modified SV40 viral genome construct into capsids formed from co-transfecting the papilloma virus L1 and L2 genes into mammalian cells;
- wherein the probe includes the luciferase marker gene of SEQ ID NO:
- 10;
- and
- wherein the modified SV40 viral genome also comprises an SV40 virus late promoter.
2 more elements
A method for detecting circulating tumor cells (CTCs) in a patient using a probe, the method comprising:
- collecting blood from a patient;
- isolating nucleated cells from the blood;
- preparing a mixture that includes the isolated nucleated cells from the blood and a probe for detecting CTCs, wherein the probe comprises a modified SV40 viral genome having a SV40 virus late promoter, a modified capsid formed from the L1 and L2 capsid proteins of a papillomavirus, and containing the luciferase (luc) marker gene of SEQ ID NO:
- 10;
- and
- detecting CTCs in the mixture.
3 more elements
Dependencies are inferred from the claim text.