Enrichment of amino acids from biomass residuum

A composition comprising:

• a distillers' grain, a first genetically engineered organism, and a second genetically engineered organism,
• wherein the first genetically engineered organism comprises an exogenous acetolactate synthase or a nucleic acid encoding the exogenous acetolactate synthase wherein the exogenous acetolactate synthase comprises a polypeptide sequence having at least 99% sequence identity to the amino acid sequence of any one of SEQ ID NOs:
• 50-53;
• and
• an exogenous 2-ketoacid decarboxylase or a nucleic acid encoding the exogenous 2-ketoacid decarboxylase wherein the exogenous 2-ketoacid decarboxylase comprises a polypeptide sequence having at least 99% sequence identity to the amino acid sequence of any one of SEQ ID NOs:
• 55-58;
• and
• the first genetically engineered organism degrades one or more carbohydrates in the distillers' grain and
• the second genetically engineered organism comprises a modified alcohol dehydrogenase having increased reactivity with nicotinamide adenine dinucleotide (NADH), as compared to a wild-type alcohol dehydrogenase, wherein the modified alcohol dehydrogenase comprises a polypeptide sequence having at least 99% sequence identity to the amino acid sequence of any one of SEQ ID NOs:
• 1-5;
• or a nucleic acid encoding the modified alcohol dehydrogenase and
• a modified ketol-acid reductoisomerase having increased reactivity with NADH, as compared to a wild-type ketol-acid reductoisomerase, or a nucleic acid encoding the modified ketol-acid reductoisomerase, or a nucleic acid encoding the modified ketol-acid reductoisomerase, wherein the modified ketol-acid reductoisomerase comprises a polypeptide sequence having at least 99% sequence identity to the amino acid sequence of any one of SEQ ID NOs:
• 6-7;
• and
• the second genetically engineered organism degrades one or more amino acids, proteins, or peptides in the distillers' grain;
• and the composition further comprising:
• about 10% total to about 25% total of valine, about 10% total to about 20% total of glutamic acid, about 5% total to about 15% total of proline, about 5% total to about 15% total of alanine, about 5% total to about 15% total of aspartic acid, about 3% total to about 10% total of tyrosine, about 3% total to about 10% total of glycine, and about 3% total to about 10% total of histidine, or a salt of any of these;
• all percentages being on a dry weight basis.