Phospholipase C mutant with high enzyme activity
AssigneeWilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd.
InventorsYaoji Xuan, Qiwen Niu, Zhengjun Xu
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Abstract
Provided in the present invention is a phospholipase C mutant with high enzyme activity, a polypeptide with the sequence as shown in SEQ ID NO:7, or a polypeptide derived from the phospholipase C formed by performing substitution, deletion or addition of one or a plurality of amino acids on the polypeptide of SEQ ID NO:7 while retaining the phospholipase C activity provided by SEQ ID NO:7. The phospholipase C mutant of the present invention can improve degumming efficiency and increase the yield of diacylglycerol (DAG) during degumming.
Claims17
An isolated polypeptide having at least 95% sequence identity to SEQ ID NO:4, wherein the amino acid residues of the isolated polypeptide that correspond to amino acids 6, 8, 10 and 104 of SEQ ID NO:
- 4 are proline, valine, serine and serine, respectively.
Dependencies are inferred from the claim text.