Psicose-6-phosphate phosphatase, composition for producing psicose including said enzyme, method for producing psicose using said enzyme
AssigneeCj Cheiljedang Corporation
InventorsByung-Sam Son, Hyun Kug Cho, Sung Jae Yang, Seong Bo Kim, Seung Hwan Kim, Hyun June Park
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Abstract
The present application relates to a psicose-6-phosphate phosphatase comprising motif A and motif B, a composition for producing D-psicose comprising the enzyme, and a method for producing D-psicose using the enzyme.
Claims15
A method for producing D-psicose comprising contacting a psicose-6-phosphate phosphatase, a microorganism expressing the psicose-6-phosphate phosphatase or a culture of the microorganism expressing the psicose-6-phosphate phosphatase with D-psicose-6-phosphate to convert the D-psicose-6-phosphate to D-psicose;
- wherein the psicose-6-phosphate phosphatase comprises:
- i) a motif A comprising at least one sequence selected from the group consisting of SEQ ID NOS:
- 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, and 91;
- ii) a motif B comprising a sequence having at least 90% sequence identity to at least one sequence selected from the group consisting of SEQ ID NOS:
- 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102,103, 104, 105, 106, 107, and 108;
- and
- iii) A remainder sequence comprising remainder having at least 85% sequence identity to the amino acids sequence other than motif A and/or motif B in any sequence selected from the group consisting of SEQ ID NOS:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20.
A method for producing D-psicose comprising contacting (a) enzymes of (i) a psicose-6-phosphate phosphatase, (ii) a D-fructose-6-phosphate-3-epimerase, (iii) a D-glucose-6-phosphate-isomerase, (iv) a phosphoglucomutase or a glucokinase and (v) one or more selected from the group consisting of an α-glucan phosphorylase, a starch phosphorylase, a maltodextrin phosphorylase, a sucrose phosphorylase, an α-amylase, a pullulanase, an isoamylase, a glucoamylase and a sucrase, (b) microorganisms expressing the enzymes listed in (a), or (c) a culture of microorganisms expressing the enzymes listed in (a) with (i) one or more selected from the group consisting of starch, maltodextrin, and sucrose, and (ii) a phosphate wherein the psicose-6-phosphate phosphatase comprises:
- i) a motif A comprising at least one sequence selected from the group consisting of SEQ ID NOS:
- 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, and 91;
- ii) a motif B comprising a sequence having at least 90% sequence identity to at least one sequence selected from the group consisting of SEQ ID NOS:
- 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, and 108;
- and
- iii) A remainder sequence comprising remainder having at least 85% sequence identity to the amino acids sequence other than motif A and/or motif B in any sequence selected from the group consisting of SEQ ID NOS:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20.
Dependencies are inferred from the claim text.