Lettuce with increased shelf life
AssigneeGreenvenus, LLC
InventorsSekhar Boddupalli, Arianne Tremblay, Eric Aasen, Jyoti R. Rout, Zhongsen Li
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Abstract
The present application is directed to lettuce plants comprising a mutation in each of at least two different PPO genes, where said mutations reduce the activity of PPO protein compared to a wild type lettuce plant. The present application is also directed to methods for making such lettuce plants.
Claims15
A genetically modified mutant lettuce plant comprising at least one or more non-naturally occurring mutations in each of at least a PPO-E gene encoding a PPO-E protein having at least 99% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:10 and a PPO-S gene encoding a PPO-S protein having at least 98% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:28, wherein said mutations eliminate or reduce activity of said PPO-E and PPO-S proteins by at least 30% in said genetically modified mutant lettuce plant as compared to a wild type lettuce plant of a same variety, wherein said mutations comprise deletions or insertions of nucleotides in the coding sequences of (i) SEQ ID NO:
- 9 (PPO-E) which encodes SEQ ID NO:
- 10 and (ii) SEQ ID NO:
- 27 (PPO-S) which encodes SEQ ID NO:
- 28, and wherein the genetically modified mutant lettuce plant at harvest comprises one or more characteristics comprising reduced tip burn;
- higher levels of polyphenolics;
- higher levels of vitamin A;
- higher levels of beta-carotene;
- higher levels of vitamin C;
- or higher levels of vitamin K as compared to said wild type lettuce plant of the same variety lacking said mutations.
A method of making a genetically modified mutant lettuce plant with reduced PPO activity, said method comprising:
- introducing at least one or more a non-naturally occurring mutations into each of at least a PPO-E gene encoding a PPO-E protein having at least 99% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:10 and a PPO-S gene encoding a PPO-S protein having at least 98% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:28 of a lettuce plant, wherein said mutations reduce the activity of said PPO-E and PPO-S proteins by at least 30% in said genetically modified mutant lettuce plant as compared to a wild type lettuce plant of a same variety, wherein said mutations comprise deletions or insertions of nucleotides in the coding sequences of (i) SEQ ID NO:
- 9 (PPO-E) which encodes SEQ ID NO:
- 10 and (ii) SEQ ID NO:
- 27 (PPO-S) which encodes SEQ ID NO:
- 28, and wherein said genetically modified mutant lettuce plant at harvest comprises one or more characteristics comprising reduced tip burn;
- higher levels of polyphenolics;
- higher levels of vitamin A;
- higher levels of beta-carotene;
- higher levels of vitamin C;
- or higher levels of vitamin K as compared to a said wild type lettuce plant of the same variety lacking said mutations.
Dependencies are inferred from the claim text.