Inducible promoter for gene expression and synthetic biology
AssigneesInstitut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement, Universite Libre de Bruxelles, Universite De Liege
InventorsJean-Marc Nicaud, Marion Trassaert, Stephane Thomas, Patrick Fickers, Marie Vandermies, Frederic Carly
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Abstract
The invention is related to an inducible promoter for improved and regulated gene expression, useful in synthetic biology and metabolic engineering. In particular, the present invention relates to a nucleotide sequence comprising the regulatory regions of an erythritol- and erythrulose-inducible promoter in yeast and uses thereof in an expression system thus allowing an improved and regulated gene expression and production of gene product.
Claims17
A method for expressing a gene or producing an encoded protein, comprising the steps of
- a) growing in vitro, in a culture medium, a host cell comprising
- an erythritol-inducible promoter sequence, an erythrulose-inducible promoter sequence or an erythritol- and erythrulose-inducible promoter sequence functional in yeast comprising a core promoter, and a nucleotide sequence comprising tandem repeats of a sequence according to formula (I) and/or tandem repeats of a sequence according to formula (II), wherein:
- formula (I) is
- CGGNNX1CNNNANNX2GNNAAGNCG (I)
- with N being any nucleotide
- with X1 and X2 being any nucleotide or no nucleotide,
- formula (II) is
- ANTTNNNTTTCCNNATNNGG (II)
- with N being any nucleotide,
- operably linked to a polynucleotide sequence encoding a gene product to be transcribed from said inducible promoter and
- b) adding erythritol and/or erythrulose to the culture medium.
Dependencies are inferred from the claim text.