Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof
AssigneeDsm IP Assets B.V.
InventorsKirk E. Apt, Leslie Richter, David Simpson, Ross Zirkle
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Abstract
The present invention is directed to isolated nucleic acid molecules and polypeptides of thraustochytrid polyunsaturated fatty acid (PUFA) synthases involved in the production of PUFAs, including PUFAs enriched in docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof. The present invention is directed to vectors and host cells comprising the nucleic acid molecules, polypeptides encoded by the nucleic acid molecules, compositions comprising the nucleic acid molecules or polypeptides, and methods of making and uses thereof.
Claims11
A recombinant nucleic acid molecule comprising a polynucleotide sequence at least 90% identical to SEQ ID NO:1 and a transcription control sequence, wherein the polynucleotide sequence encodes a polypeptide comprising beta-keto acyl-ACP synthase (KS) activity, malonyl-CoA:
- ACP acyltransferase (MAT) activity, acyl carrier protein (ACP) activity, ketoreductase (KR) activity, and beta-hydroxyacyl-ACP dehydrase (DH) activity, wherein said polynucleotide sequence is heterologous to said transcription control sequence.
A recombinant nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide and a transcription control sequence, wherein the polypeptide comprises an amino acid sequence at least 90% identical to SEQ ID NO:2, wherein the polypeptide comprises beta-keto acyl-ACP synthase (KS) activity, malonyl-CoA:
- ACP acyltransferase (MAT) activity, acyl carrier protein (ACP) activity, ketoreductase (KR) activity, and beta-hydroxyacyl-ACP dehydrase (DH) activity, wherein said polynucleotide sequence is heterologous to said transcription control sequence.
A method to produce at least one polyunsaturated fatty acid (PUFA), comprising:
- expressing a PUFA synthase gene in a host cell under conditions effective to produce PUFA, wherein the PUFA synthase gene comprises a nucleic acid molecule comprising a polynucleotide sequence at least 90% identical to SEQ ID NO:
- 1 or a polynucleotide sequence encoding a polypeptide which comprises an amino acid sequence at least 90% identical to SEQ ID NO:2, wherein said nucleic acid molecule is heterologous to the host cell, wherein said polynucleotide sequence encodes a PUFA synthase polypeptide comprising beta-keto acyl-ACP synthase (KS) activity, malonyl-CoA:
- ACP acyltransferase (MAT) activity, acyl carrier protein (ACP) activity, ketoreductase (KR) activity, and beta-hydroxyacyl-ACP dehydrase (DH) activity, and wherein at least one PUFA is produced.
A method to produce lipids enriched for docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof as compared to a host cell not expressing a polyunsaturated fatty acid (PUFA) synthase gene as claimed, comprising:
- expressing a PUFA synthase gene in a host cell under conditions effective to produce lipids, wherein the PUFA synthase gene comprises a nucleic acid molecule comprising a polynucleotide sequence at least 90% identical to SEQ ID NO:1 or a polynucleotide sequence encoding a polypeptide which comprises an amino acid sequence at least 90% identical to SEQ ID NO:
- 2, wherein said nucleic acid molecule is heterologous to the host cell, wherein said polynucleotide sequence encodes a PUFA synthase polypeptide comprising beta-hydroxyacyl-ACP dehydrase (DH) activity and enoyl-ACP reductase (ER) activity, beta-keto acyl-ACP synthase (KS) activity, malonyl-CoA:
- ACP acyltransferase (MAT) activity, acyl carrier protein (ACP) activity, ketoreductase (KR) activity, and beta-hydroxyacyl-ACP dehydrase (DH) activity, and wherein lipids enriched with DHA, EPA, or a combination thereof are produced.
Dependencies are inferred from the claim text.