Catalyst-free surface functionalization and polymer grafting
AssigneeIllumina, Inc.
InventorsLorenzo Berti, Andrew A. Brown, Wayne N. George
Life of this patent
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Abstract
Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.
Claims18
A method of preparing a first surface of a substrate, the method comprising:
- providing a substrate having a first surface comprising surface-bound silane or silane derivative, wherein the silane or silane derivative comprises a first plurality of unsaturated moieties selected from the group consisting of norbornene, heteronorbornene, norbornene derivatives, and optionally substituted variants and combinations thereof, and wherein the first plurality of unsaturated moieties are covalently attached to the silane or silane derivative, optionally through linkers;
- covalently attaching a functionalized molecule comprising functional groups to the silane or silane derivative by reacting the functional groups of said functionalized molecule with the first plurality of unsaturated moieties to form a coating layer;
- contacting oligonucleotides comprising a second plurality of unsaturated moieties with the functionalized molecule, wherein the second plurality of unsaturated moieties are selected from the group consisting of cycloalkenes, cycloalkynes, heterocycloalkenes, heterocycloalkynes, alkynes, and optionally substituted variants and combinations thereof;
- reacting the second plurality of unsaturated moieties of the oligonucleotides with the functional groups of the functionalized molecule to form covalent bonding, wherein the functionalized molecule comprises poly(N-(5-azidoacetamidylpentyl) acrylamide-co-acrylamide) (PAZAM).
Dependencies are inferred from the claim text.