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AssigneeLimited Liability Company “Urspharm”
InventorsVladimir Glebovich Lunin, Sergei Mihailovich Yudin, Vyacheslav Viktorovich Rechetnik, Vali-Magomed Kadievich Magataev
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The present invention relates to genetic engineering, biotechnology, immunology, and microbiology. Recombinant GBD-SSTad-SSTad protein and a method for preparation thereof on glucan are described, including the binding of GBD-SSTad-SSTad protein in cell extracts of E. coli BL21 [pGBD-SSTad-SSTad] strain to an alpha-glycan-containing sorbent due to affinity interaction during the incubation procedure, subsequent washing from unbound bacterial proteins and isolation of the desired product. The invention also relates to an immunogenic composition and a kit comprising said peptide. The invention also relates to the use of the obtained protein to increase the number of maturing follicles and improve the quality of sperm. This technical result, an increase in the number of maturing follicles and an improvement in the quality of sperm is based on the use of the KNFFWKTFTS peptide, which is part of a recombinant protein that is capable of eliciting autoantibodies to somatostatin.
A recombinant GBD-SSTad-SSTad protein having a molecular weight of 39.5 kDa, comprising 2 fragments of the SST protein having the sequence SEQ ID NO 1, a spacer having the sequence SEQ ID NO 2 and a spacer having the sequence SEQ ID NO 3, alpha-glucan binding domain from Streptococcus mutans having the sequence SEQ ID NO 4, which protein is encoded by the nucleotide sequence of the GBD-SSTad-SSTad gene SEQ ID NO 5.
Dependencies are inferred from the claim text.