Device including a hydrophilic, non-ionic coating for size exclusion chromatography

A method of modifying a fluidic flow path disposed within an interior of a fluidic system, the fluidic flow path having a wetted surface comprising hydroxyl groups, the method comprising:

• a. contacting the fluidic flow path with a vaporized organosilane reagent of Formula I
• wherein:
• R1, R2, R3, R4, R5, and R6 are each independently selected from the group consisting of (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, and halo;
• and
• Z is (C1-C20)alkyl, —[O(CH2)2O]1-20, —[(C1-C10)NH(CO)NH(C1-C10)]1-20—, or —[(C1-C10)alkylphenyl(C1-C10)alkyl]1-20;
• to form a first coating deposited on at least a portion of the wetted surface of the fluidic flow path;
• and
• b. contacting the fluidic flow path having the first coating deposited thereon with a solution comprising an organosilane reagent of Formula II
• wherein:
• R7, R8, and R9 are each independently selected from the group consisting of C1-C6 alkyl, (C1-C6)alkenyl, —NHR10, —NR10R11, OH, OR10, and halo;
• R10 and R11 are each independently selected from C1-C6 alkyl;
• Y is —(C1-C6)alkyl —OR12, —[O(C1-C3)alkyl]1-10O(C1-C6)alkyl, —[O(C1-C3)alkyl]1-10OH or phenyl, wherein said (C1-C6)alkyl is substituted with one or more halo or one or more functional groups selected from the group consisting of OH, amino, (C1-C6)alkylamino, di(C1-C6)alkylamino, cyano, —C(O)NH2, sulfonic acid, and carboxyl, and wherein said phenyl is optionally substituted with one or more groups selected from the group consisting of (C1-C3)alkyl, OH, halo, cyano, —C(O)NH2, and carboxyl;
• R12 is —(C1-C3)alkyloxirane, —(C1-C3)alkyl-3,4-epoxycyclohexyl, or —(C1-C4)alkylOH;
• to form a second coating on at least a portion of the wetted surface of the fluidic flow path, and in direct contact with the first coating.