Pipette tip and uses and methods thereof

A pipette tip comprising a constituent (A) and a constituent (B), the pipette tip having:

• an open upper end configured to fit to an end of a pipette;
• an open lower end configured to aspirate and release a liquid sample;
• a longitudinal axis spanning a length from the open upper end to the open lower end;
• an inner surface;
• a first reagent layer deposited on a first region of the inner surface;
• and
• a second reagent layer deposited on a second region of the inner surface;
• wherein the first reagent layer comprises constituent (A) and the second reagent layer a comprises constituent (B),
• constituent (A) being physically confined and releasably coupled to the first region and being separate from constituent (B), and
• constituent (B) being physically confined and releasably coupled to the second region and being separate from constituent (A),
• wherein:
• (a) the first region and the second region are non-overlapping,
• (b) constituent (A) is different than constituent (B);
• (c) constituent (A) and constituent (B) are configured to form a diagnostic composition upon their release into the liquid sample from the first region and the second region;
• and
• (d) wherein each of the constituents (A) and (B) is an essentially dry formulation or a dry formulation deposited on the inner surface;
• (e) the first region and the second region are juxtaposed opposite the longitudinal axis from each other.