Method of testing a blood for macrolide immunosuppressant
AssigneeFujirebio Inc.
InventorsMasaya Toyonaga, Yoshiyuki Hotta
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Abstract
The present invention provides a method of testing a blood for a macrolide immunosuppressant conveniently and/or highly accurately. Specifically, the present invention provides a method of testing a blood for a macrolide immunosuppressant, including: (a) treating a blood sample containing a macrolide immunosuppressant or a metabolite thereof having a macrolide structure with an acid or an alkali; and (b) measuring a concentration of the macrolide immunosuppressant or the metabolite in the blood sample by using an antibody.
Claims2
A method of testing a blood for a macrolide immunosuppressant that is tacrolimus, the method comprising:
- (a) treating a blood sample comprising tacrolimus with an acidic buffer having a pH in a range of 2.0 to 4.5 to form an acidic blood sample containing the tacrolimus, and wherein the acidic blood sample containing the tacrolimus does not contain a volatile organic solvent;
- and
- (b) measuring a concentration of the tacrolimus in the acidic blood sample by contacting the acidic blood sample with an antibody that specifically binds to the tacrolimus,
- and the antibody is IgG, IgM, or an antibody fragment thereof, and immobilized to a solid phase.
Dependencies are inferred from the claim text.