Method and system for buoyant-particle-assisted cell therapy

A method for buoyancy-assisted production of cells for a cell therapy, the method comprising:

• initiating a 1st selection process, the 1st selection process comprising:
• combining T cells and a 1st set of buoyant particles, wherein the 1st set of buoyant particles are functionalized with moieties for binding to and forming a complex with the T cells to produce first bound T cells;
• and
• initiating a cell activation and expansion process comprising a 2nd selection process, the cell activation and expansion process comprising:
• producing second bound T cells by combining a set of activators factors, a 2nd set of buoyant particles, and the T cells within a container, wherein the set of activation factors are antibodies, wherein the 2nd set of buoyant particles are functionalized with moieties for binding to and forming a complex with the antibodies, wherein in at least a portion of the second bound T cells, a single T cell is bound to multiple buoyant particles of the 2nd set of buoyant particles, wherein the second bound T cells aggregate at a superior region of the container;
• culturing the second bound T cells in the container to produce daughter cells from the second bound T cells;
• increasing a spatial separation of the daughter cells relative to the second bound T cells, thereby preventing exhaustion of the daughter cells due to exposure of the daughter cells to the antibodies, wherein the daughter cells aggregate at an inferior surface of the container;
• and
• collecting the daughter cells, wherein the daughter cells are used in the cell therapy.