Arrays of integrated analytical devices
AssigneePacific Biosciences of California, Inc.
InventorsAnnette Grot, Ravi Saxena, Paul Lundquist
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Abstract
Arrays of integrated analytical devices and their methods for production are provided. The arrays are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices allow the highly sensitive discrimination of optical signals using features such as spectra, amplitude, and time resolution, or combinations thereof. The devices include an integrated diffractive beam shaping element that provides for the spatial separation of light emitted from the optical reactions.
Claims15
An array of integrated analytical devices, each device comprising:
- a nanowell;
- a nanoscale emission volume within the nanowell;
- a detector layer optically coupled to the nanoscale emission volume;
- a lens element layer disposed between the nanowell and the detector layer;
- and
- an aperture layer disposed between the nanowell and the detector layer;
- wherein a signal light is emitted from the nanoscale emission volume by an emitter within the nanoscale emission volume;
- wherein the lens element layer directs the signal light from the nanoscale emission volume to a sensing region on the detector layer;
- and
- wherein the aperture layer reduces transmission of a non-signal light to the sensing region.
Dependencies are inferred from the claim text.