Size-tunable synthetic particles with tunable optical properties and methods for using the same for immune cell activation
AssigneeSlingshot Biosciences, Inc.
InventorsDaixuan Zhang, Solomon Stonebloom, Juan Armas, Harini Kethar, Sunil Thomas, Martina De Geus
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Abstract
A hydrogel particle, comprising a matrix comprising a polymerized monomer, said matrix comprising a plurality of micropores and a plurality of macropores, and one or more immunostimulatory biomolecules selected from the group consisting of an anti-CD3 antibody or antigen-binding fragment thereof, an anti-CD28 antibody or antigen-binding fragment thereof, and combinations thereof.
Claims98
A method of inducing an immune cell response, comprising:
- contacting an immune cell with a particle comprising:
- a matrix comprising a polymerized monomer having a plurality of micropores and a plurality of macropores;
- and
- one or more immunostimulatory or co-stimulatory biomolecules,
- wherein the immune cell response comprises activation and/or expansion of the immune cell, and wherein an average diameter of the plurality of macropores is between about 200 nm and about 2 μm.
A method for calibrating a cytometric device for analysis of a target cell, comprising,
- inserting into the device a hydrogel particle comprising (i) a matrix comprising a polymerized monomer having a plurality of micropores and a plurality of macropores;
- and (ii) one or more immunostimulatory or co-stimulatory biomolecules, wherein the particle has at least one optical or morphological property substantially similar to the target cell;
- measuring the at least one optical or morphological property of the particle using the cytometric device;
- and
- calibrating the cytometric device based on the optical or morphological property measurement of the particle.
A method for detecting a target cell in a sample, comprising,
- inserting into a cytometric device a hydrogel particle comprising (i) a matrix comprising a polymerized monomer having a plurality of micropores and a plurality of macropores;
- and (ii) one or more immunostimulatory or co-stimulatory biomolecules, wherein the particle has at least one optical or morphological property substantially similar to the target cell;
- measuring the at least one optical or morphological property of the particle using the cytometric device;
- inserting a sample in the cytometric device comprising a plurality of cells;
- measuring the at least one optical or morphological property of individual cells of the plurality;
- and
- determining, based on the optical or morphological property measurement, whether the target cell or plurality thereof is present in the sample.
A method of inducing an immune cell response, comprising:
- contacting an immune cell with a particle comprising:
- a matrix comprising a polymerized monomer having a plurality of micropores and a plurality of macropores;
- and
- one or more immunostimulatory or co-stimulatory biomolecules,
- wherein the immune cell response comprises activation and/or expansion of the immune cell, and
- wherein the immune cell response from contacting the immune cell with the particle is higher than the immune cell response from a control immune cell contacted with an otherwise identical particle lacking the macropores.
A method of inducing an immune cell response, comprising:
- contacting an immune cell with a particle comprising:
- a matrix comprising a polymerized monomer having a plurality of micropores and a plurality of macropores;
- and
- one or more immunostimulatory or co-stimulatory biomolecules,
- wherein the immune cell response comprises activation and/or expansion of the immune cell, and
- wherein the plurality of micropores are formed during monomer polymerization.
A method of inducing an immune cell response, comprising:
- contacting an immune cell with a particle comprising:
- a matrix comprising a polymerized monomer having a plurality of micropores and a plurality of macropores;
- and
- one or more immunostimulatory or co-stimulatory biomolecules,
- wherein the immune cell response comprises activation and/or expansion of the immune cell, and
- wherein the particle has a diameter of between about 1 μm and about 25 μm.
Dependencies are inferred from the claim text.