RNA polymerase variants
AssigneeModernaTX, Inc.
InventorsAmy E. Rabideau, Athanasios Dousis, Kanchana Ravichandran, Elissa Hobert
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Abstract
The present disclosure provides, in some aspects, variant RNA polymerases, the use of which increases transcription efficiency while reducing the number of double-stranded RNA contaminates and run-on transcripts produced during an in vitro transcription reaction.
Claims12
A method of performing an in vitro transcription (IVT) reaction, comprising combining a deoxyribonucleic acid (DNA) template with a T7 ribonucleic acid (RNA) polymerase variant, nucleoside triphosphates, and buffer under conditions that result in the production of to produce an RNA transcript, wherein the T7 RNA polymerase variant comprises an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO:
- 1 modified to comprise an amino acid substitution at position.
Dependencies are inferred from the claim text.