Methods of producing RPE cells
AssigneeBiolamina AB
InventorsOuti Hovatta, Karl Tryggvason
Life of this patent
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Abstract
The present disclosure relates to the use of laminin-521 in obtaining retinal pigment epithelium (RPE) cells. Pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in totally defined and xeno-free conditions. A first cell culture medium contains a growth factor, and a second cell culture medium does not contain growth factor. The stem cells are first exposed to the first cell culture medium, then exposed to the second cell culture medium for a longer time period. After a number of weeks, clinical grade RPE cells are obtained from the stem cells.
Claims14
A method of obtaining retinal pigment epithelium (RPE) cells, comprising:
- culturing one or more stem cells on a substrate comprising a laminin, wherein the laminin is an intact protein or a protein fragment, and wherein the laminin is laminin-521 or laminin-511;
- exposing the stem cells to a first cell culture medium that contains a growth factor;
- after a first time period, removing the first cell culture medium and exposing the stem cells to a second cell culture medium that does not contain the growth factor;
- and
- periodically changing the second cell culture medium to obtain the RPE cells.
Dependencies are inferred from the claim text.