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US 12,195,749 B2Granted
Nucleic acid-guided nucleases
AssigneeInscripta, Inc.
InventorsAndrew Garst, Ryan T. Gill, Tanya Elizabeth Warnecke Lipscomb
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Abstract
Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.
Claims19
A nucleic acid-guided nuclease system comprising:
- (a) a nucleic acid-guided nuclease encoded by a nucleic acid molecule, wherein said nucleic acid molecule encoding said nucleic acid-guided nuclease has at least 85% sequence identity to the nucleotide sequences of SEQ ID NO:
- 44 or SEQ ID NO:
- 24 and encodes an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 4;
- (b) an engineered guide nucleic acid sequence capable of complexing with the nucleic acid-guided nuclease, wherein the engineered guide nucleic acid sequence is configured to hybridized to a TTTN sequence of a target region in a genome of a cell;
- (c) an editing sequence having a change in sequence relative to the sequence of a target region in a genome of a cell;
- wherein the nucleic acid-guided system results in a genome edit in the target region in the genome of the cell facilitated by the nucleic acid-guided nuclease, the engineered guide nucleic acid, and the editing sequence.
4 more elements
Dependencies are inferred from the claim text.