Delivery across cell plasma membranes
AssigneeAvectas Limited
InventorsMichael Maguire, Shirley O'Dea
Life of this patent
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Abstract
Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.
Claims30
An apparatus for delivering a payload across a plasma membrane of a cell, the apparatus comprising:
- a pneumatic generator configured to produce a gas under a pressure;
- an atomizer operatively coupled to the pneumatic generator, wherein the atomizer is oriented to contact a population of cells with an aqueous solution;
- a valve between the pneumatic generator and atomizer, the valve switchable between a closed position for preventing the gas under the pressure from activating the atomizer and an open position for allowing the gas under the pressure to activate the atomizer to produce colloidal droplets from the aqueous solution;
- and
- a reservoir containing a volume of the aqueous solution, the aqueous solution including the payload and an alcohol at greater than 2 percent concentration (v/v), less than 46 mM salt, less than 121 mM sugar, and less than 19 mM buffering agent, wherein the salt is present in the aqueous solution and selected from a group consisting of NaCl, KCl, Na2HPO4, C2H3O2NH4, and KH2PO4.
Dependencies are inferred from the claim text.