Creation of herbicide resistant gene and use thereof
AssigneesInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, China Agricultural University
InventorsCaixia Gao, Linjian Jiang
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Abstract
The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for creating novel herbicide resistant plants by base editing techniques and a method for screening endogenous gene mutation sites capable of conferring herbicide resistance in plants. The invention also relates to the use of the identified endogenous gene mutantation sites in crop breeding.
Claims16
A nucleotide sequence encoding an ACCase mutant, the nucleotide sequence comprising at least one nucleotide substitution resulting in at least one amino acid mutation selected from the group consisting of S1768F, Q1828*, P1829F, and A2025T, and combinations thereof,
- wherein the amino acid position refers to SEQ ID NO:
- 1,
- wherein said ACCase mutant confers herbicide resistance to a plant.
A nucleotide sequence encoding an ALS mutant, the nucleotide sequence comprising at least one nucleotide substitution resulting in an amino acid mutation at one or more positions selected from 655, 659, and combinations thereof,
- wherein the amino acid position refers to SEQ ID NO:
- 2,
- wherein said mutant confers herbicide resistance to a plant, and wherein the amino acid mutation at position 655 is selected from the group consisting of G655D, G655S, and G655N.
Dependencies are inferred from the claim text.