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US 12,195,736 B2Granted
Methods for plant transformation using spectinomycin selection
AssigneeMonsanto Technology LLC
InventorsBrian J. Martinell, Michael W. Petersen, David A. Somers, Yuechun Wan, Edward Williams, Xudong Ye
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Abstract
The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
Claims19
A method for producing a transgenic plant comprising:
- (a) culturing an Agrobacterium comprising a DNA sequence encoding an aminoglycoside adenyltransferase (aadA) sequence inside the T-DNA sequence and a bacterial selection gene other than aadA, in a medium comprising a bacterial selection compound other than spectinomycin;
- (b) contacting an explant with the Agrobacterium;
- (c) co-culturing the explant with the Agrobacterium;
- and
- (d) regenerating the plant on media containing spectinomycin, wherein the media simultaneously selects for the transformed explant and kills the Agrobacterium in a single media;
- wherein the aadA sequence comprises SEQ ID NO:2.
3 more elements
Dependencies are inferred from the claim text.