Methods of producing T cell populations enriched for stable regulatory T-cells
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsYong Chan Kim, Ethan Shevach
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Abstract
The present invention provides methods for producing cell populations enriched for stable, regulatory T cells (Tregs). In particular, the invention relates to methods for culturing T cells such that the final culture is enriched for stable, regulatory T cells. It also relates to methods for stabilizing regulatory T cells. Also provided are compositions enriched for stable, regulatory T cells, which are useful for treating individuals in need of such treatment. The methods and compositions disclosed herein can also be used to treat an individual suffering from an immune-mediated disease.
Claims7
A method of treating multiple sclerosis in an individual in need thereof, comprising administering to the individual an effective amount of a composition comprising a population of isolated, human regulatory T cells, wherein the regulatory T cells are CD4+Foxp3+Helios+CD25+CD127lo or CD4+Foxp3+Helios+CD25+CD127−, wherein the regulatory T cells comprise a plurality of cytoplasmic oligodeoxynucleotides (ODN), wherein the ODN comprise a phosphorothioate backbone, and wherein the ODN are between 11-49 nucleotides in length, wherein each ODN comprises repeats of CpG, TTAGGG, NNNGGG, or NNNNNN, wherein each N in each ODN is a completely random nucleotide, and wherein there is an equal probability that any one of adenine, guanine cytosine, and thymidine will be present at each N.
Dependencies are inferred from the claim text.