3D printed, high-throughput microelectrode array
AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsSwaminathan Rajaraman, Avra Kundu, Adam Rozman, Jorge Manrique Castro
Life of this patent
Estimating the term…
Abstract
A high-throughput, three-dimensional microelectrode array for in vitro electrophysiological applications includes a 3D printed well plate having a top face and bottom face, and a plurality of culture wells formed on the top face of the well plate. Each culture well includes a plurality of vertical microchannels on the top face and microtroughs formed on the bottom face and communicating with the microchannels. A conductive paste fills the microtroughs and the microchannels and forms self-isolated microelectrodes in each culture well and conductive traces that communicate with the self-isolated microelectrodes.
Claims18
A high-throughput, three-dimensional (3D) microelectrode array system for in vitro electrophysiological applications, comprising:
- a 3D printed well plate having a top face and bottom face;
- a plurality of culture wells formed on the top face of the well plate, and including a plurality of 3D printed, hollow, vertically extending microchannels having an inner diameter of about 570 to 630 micrometers formed within each culture well that extend upward from the top face to form microtowers having a height of about 595 to 605 micrometers;
- 3D printed microtroughs formed on the bottom face and communicating with the microchannels;
- and
- a conductive paste filling the microtroughs and the microchannels and forming on the top face a plurality of self-isolated microelectrodes in each culture well and on the bottom face conductive traces that communicate with the plurality of self-isolated microelectrodes, wherein each microelectrode includes a mushroom shaped, electroplated top contact section that includes an obtuse angle with the top of the microtower.
A high-throughput, three-dimensional (3D) microelectrode array system for in vitro electrophysiological applications, comprising:
- a 3D printed well plate having a top face and bottom face;
- a plurality of cylindrical culture wells formed on the top face of the well plate, and including a plurality of 3D printed, hollow, vertically extending microchannels having an inner diameter of about 570 to 630 micrometers formed within each cylindrical culture well, wherein said vertical microchannels are arranged in an ordered or random array within each of the plurality of cylindrical culture wells, and extend upward from the top face to form microtowers having a height of about 595 to 605 micrometers;
- 3D printed microtroughs formed on the bottom face opposite each culture well and communicating with respective microchannels formed within a respective opposite culture well;
- and
- a conductive paste filling the microtroughs on the bottom face and the microchannels on the top face and forming on the top face a plurality of self-isolated microelectrodes in each culture well and on the bottom face conductive traces that communicate with the plurality of self-isolated microelectrodes, wherein each microelectrode includes a mushroom shaped, electroplated top contact section that includes an obtuse angle with the top of the microtower.
A high-throughput, three-dimensional (3D) microelectrode array system for in vitro electrophysiological applications, comprising:
- a 3D printed well plate having a top face and bottom face;
- a plurality of cylindrical culture wells formed on the top face of the well plate and arranged in an ordered or random array, and including a plurality of 3D printed, hollow, vertically extending microchannels having an inner diameter of about 570 to 630 micrometers formed within each of the plurality of cylindrical culture wells, wherein said 3D printed vertical microchannels are arranged in an ordered or random array within each of the cylindrical culture wells, and extend upward from the top face to form microtowers having a height of about 595 to 605 micrometers;
- 3D printed microtroughs formed on the bottom face opposite each culture well and communicating with respective microchannels formed within a respective opposite culture well;
- and
- a conductive paste filling the microtroughs and the microchannels and forming on the top face a plurality of self-isolated microelectrodes in each culture well and conductive traces that communicate with the self-isolated microelectrodes, wherein the conductive traces terminate into contact pads configured to interface with an electrophysiological circuit component, wherein each microelectrode includes a mushroom shaped, electroplated top contact section that includes an obtuse angle with the top of the microtower.
Dependencies are inferred from the claim text.