Perfusion bioprocessing system and method of operating the same

An automatic perfusion bioprocessing system comprising:

• a bioreactor;
• a recirculation flow path provided with at least one first feed flow control device and at least one second feed control device;
• a first tangential flow filter coupled to the bioreactor via the recirculation flow path;
• a second tangential flow filter coupled to the bioreactor via the recirculation flow path;
• a permeate flow path, wherein the first tangential flow filter and the second tangential flow filter are coupled to the permeate flow path;
• a retentate flow path, wherein the first tangential flow filter and the second tangential flow filter are coupled to the retentate flow path;
• and
• a control unit coupled to the at least one first feed flow control device and the at least one second feed control device;
• wherein the control unit is configured to control the at least one first feed flow control device for controlling a flow of a first quantity of a process fluid from the bioreactor to the first tangential flow filter via the recirculation flow path;
• wherein the control unit is further configured to control at least one of the at least one first feed flow control device and the at least one second feed control device for controlling a flow of a second quantity of the process fluid from the bioreactor to the second tangential flow filter via the recirculation flow path, automatically bypassing the
• first tangential flow filter upon determination of a clogged condition or a substantially clogged condition of the first tangential flow filter;
• wherein the recirculation flow path is configured to recirculate the process fluid from the bioreactor, through one or both of the first tangential flow filter and the second tangential flow filter, back to the bioreactor;
• and
• wherein the first tangential flow filter and the second tangential flow filter are arranged in series.