Mutant fragments of OspA and methods and uses relating thereto
AssigneeValneva Austria GmbH
InventorsPär Comstedt, Markus Hanner, Urban Lundberg, Andreas Meinke, Wolfgang Schüler, Benjamin Wizel
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Abstract
The present invention relates to a polypeptide comprising a mutant fragment of an outer surface protein A (OspA), a nucleic acid coding the same, a pharmaceutical composition (particularly for use as a medicament of in a method of treating or preventing a Borrelia infection) comprising the polypeptide and/or the nucleic acid, a method of treating or preventing a Borrelia infection and a method of immunizing a subject.
Claims23
A method for producing a polypeptide comprising the following steps:
- a) introducing a vector encoding the polypeptide into a host cell;
- b) growing the host cell under conditions allowing for expression of said polypeptide;
- c) homogenizing said host cell;
- and
- d) subjecting the host cell homogenate to purification steps,
- wherein said polypeptide comprises a mutant fragment of a Borrelia outer surface protein A (OspA),
- wherein said mutant OspA fragment comprises a C-terminal domain of a Borrelia OspA, and differs from the corresponding wild-type Borrelia OspA fragment sequence at least by the introduction of at least one disulfide bond that is formed by the introduction of:
- i) one cysteine, which forms said disulfide bond with a cysteine residue present within the wild type fragment;
- or
- ii) two cysteines, which together form said disulfide bond.
A method for producing a pharmaceutical composition comprising combining a polypeptide with one or more pharmaceutically acceptable carriers or excipients,
- wherein said polypeptide comprises a mutant fragment of a Borrelia outer surface protein A (OspA),
- wherein said mutant OspA fragment comprises a C-terminal domain of a Borrelia OspA, and differs from the corresponding wild-type Borrelia OspA fragment sequence at least by the introduction of at least one disulfide bond that is formed by the introduction of
- i) one cysteine, which forms said disulfide bond with a cysteine residue present within the wild type fragment;
- or
- ii) two cysteines, which together form said disulfide bond.
Dependencies are inferred from the claim text.