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US 12,194,432 B2Granted
Chromatography resin having an anionic exchange-hydrophobic mixed mode ligand
AssigneeBio-Rad Laboratories, Inc.
InventorsChristopher Belisle, Hong Chen, Yueping Xu, Jiali Liao, Xuemei He
Life of this patent
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Abstract
Chromatography resins having mixed mode ligands and methods of using such resins are provided.
Claims28
A chromatography configuration comprising a packed column, fluidized column, expanded-bed column, monolith or porous membrane comprising a chromatography resin having the formula:
- Chromatography matrix-(X1)-L-Ar-(X2)-Y
- or a tautomer or an anionic salt thereof,
- wherein:
- X1 is a spacer;
- X2 is C1 to C5 alkyl, C3 or C5 cycloalkyl;
- L is NR8, O, or S wherein R8 is hydrogen or C1 to C6 alkyl;
- Ar is a 6- to 10-membered aryl mono or bicyclic ring optionally substituted with up to four C1 to C3 unsubstituted alkyl or C3 to C6 branched alkyl;
- and
- Y is selected from the group consisting of:
- wherein:
- R1, R2, R3, R4, R5, R6, and R7 are each independently hydrogen or C1 to C6 alkyl;
- if Y is
- R2 is optionally joined to R3 to form a 4- to 7-membered heterocycle or R3 is optionally joined to R4 to form a 4- to 7-membered heterocycle;
- if Y is
- R1 is optionally joined to R2 to form a 4- to 7-membered heterocycle and R3 is optionally joined to R4 to form a 4- to 7-membered heterocycle;
- and
- if Y is
- R5 is optionally joined to R6 to form a 4- to 7-membered heterocycle or R6 is optionally joined to R7 to form a 4- to 7-membered heterocycle.
15 more elements
A chromatography configuration comprising a chromatography resin having the formula:
- Chromatography matrix-(X1)-L-Ar-(X2)-Y
- or a tautomer or an anionic salt thereof,
- wherein:
- X1 is a spacer;
- X2 is C1 to C5 alkyl, C3 or C5 cycloalkyl or is absent;
- L is NR8, O, or S wherein R8 is hydrogen or C1 to C6 alkyl;
- Ar is a 6- to 10-membered aryl mono or bicyclic ring optionally substituted with up to four C1 to C3 unsubstituted alkyl or C3 to C6 branched alkyl;
- and
- Y is selected from the group consisting of:
- wherein R1 and R4 are each independently hydrogen or C1 to C6 alkyl and R2 is joined to R3 to form a 4- or 5-membered heterocycle;
- wherein R1 and R2 are each independently hydrogen or C1 to C6 alkyl and R3 is joined to R4 to form a 4- to 6-membered heterocycle;
- and
- wherein R1, R2, R3, and R4 are each independently hydrogen or C1 to C6 alkyl and R5 is joined to R6 to form a 4- or 5-membered heterocycle or R1, R2, R3, R4, and R5 are each independently hydrogen or C1 to C6 alkyl R6 is joined to R7 to form a 4- or 5-membered heterocycle.
10 more elements
Dependencies are inferred from the claim text.