Sunflower seed protein isolate and a process for producing the same
AssigneesAvril, Universite De Lorraine, Centre National De La Recherche Scientifique
InventorsOlivier Galet, Romain Kapel, Sara Albe Slabi
Life of this patent
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Abstract
A process for preparing a sunflower seed protein isolate and a protein isolate which is obtainable by such process. The process comprises the following steps: mixing a defatted seed meal with an aqueous NaCl solution at a basic pH; separating said solubilised protein solution from solids; diafiltering said solubilised protein solution through an ultrafiltration membrane system using an aqueous NaCl diafiltration NaCl solution and at least 2 diavolumes of said aqueous NaCl diafiltration solution, diafiltering said NaCl-diafiltered protein; concentrating said purified protein solution; and drying said purified protein concentrate to obtain a protein isolate.
Claims12
A process for preparing a sunflower protein isolate, said process comprising the following steps:
- (a) providing an at least partially defatted seed meal, said seed meal being a sunflower seed meal and having a proportion of dry matter ranging from 80 wt % to 98 wt %;
- (b) mixing said at least partially defatted seed meal with an aqueous NaCl solution at a pH of about 6 to 8, in order to solubilize proteins present in said at least partially defatted seed meal and to thus obtain a solubilized protein solution, wherein said aqueous NaCl solution has a NaCl concentration ranging from 0 to 1.2 mol·L−1;
- (c) separating said solubilized protein solution from solids therein;
- (d) diafiltering said solubilized protein solution through an ultrafiltration membrane system having a molecular weight cutoff of 1 to 100 kDa, said diafiltering step being effected using:
- an aqueous NaCl diafiltration solution having a NaCl concentration ranging from 0.1 to 0.6 mol·L−1;
- and
- at least 2 diavolumes of said aqueous NaCl diafiltration solution, to obtain a NaCl-diafiltered protein solution;
- (e) subsequently to step (d), diafiltering said NaCl-diafiltered protein solution through an ultrafiltration membrane system with a molecular weight cutoff of 1 to 100 kDa, using water, to obtain a purified protein solution;
- (f) concentrating said purified protein solution to obtain a purified protein concentrate;
- and
- (g) drying said purified protein concentrate to obtain said protein isolate;
- wherein said process does not contain a step of precipitation of said protein prior to the step (d).
Dependencies are inferred from the claim text.