Background reduction in top-down antibody analysis
AssigneeDH Technologies Development Pte. Ltd.
InventorsTakashi Baba, Pavel Ryumin
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Abstract
Method and devices for performing top down analysis of an antibody are described which involve utilizing an ion source to generate a plurality of ions from a sample containing at least one intact antibody. Further, transmitting said plurality of ions through a quadrupole rod set while applying an RF signal thereto and in the absence of a resolving DC voltage so as to preferentially transmit precursor ions having an m/z value greater than a low mass cutoff of about 1500 m/z from the quadrupole rod set to an ECD cell. The method and device can also performing an ECD reaction in the ECD cell on said precursor ions and also detect reaction products from the ECD reaction.
Claims20
A method for performing top down analysis of an antibody, comprising:
- utilizing an ion source to generate a plurality of ions from a sample containing at least one intact antibody,
- transmitting said plurality of ions through at least one quadrupole rod set while applying an adjustable RF signal thereto and without applying a resolving DC voltage to the quadrupole rod set so as to preferentially transmit precursor ions having an m/z value greater than a low mass cutoff of about 1500 m/z from the quadrupole rod set to an ECD cell;
- performing an ECD reaction in the ECD cell on said precursor ions;
- detecting reaction products from the ECD reaction.
A mass spectrometer system, comprising:
- an ion source for generating a plurality of ions from a sample containing intact antibodies;
- at least one quadrupole rod set extending along a central longitudinal axis and configured to receive said plurality of ions from said ion source through an inlet end and to transmit at least a portion of said ions through an outlet end, the quadrupole rod set comprising a first pair of elongate rods and a second pair of elongate rods disposed around and parallel to the central longitudinal axis;
- an ECD cell for receiving ions transmitted from the quadrupole rod set and within which said ions received from the quadrupole rod set are reacted with electrons to generate product ions therefrom;
- a detector for detecting said product ions;
- and
- a power system electrically coupled to the quadrupole rod set, said power system configured to provide an adjustable RF signal to the quadrupole rod set in the absence of without applying a resolving DC voltage to the quadrupole rod set so as to substantially prevent ions having a m/z value less than a low mass cutoff of about 1500 m/z from being transmitted from the quadrupole rod set to the ECD cell.
Dependencies are inferred from the claim text.