Method for discriminating sex of yellowtail group

A method for identifying sex of at least one yellowtail, comprising the steps of:

• identifying whether the 169th nucleotide residue in exon 3 of HSD17B1 gene of the at least one yellowtail is an adenine (A) residue or a guanine (G) residue,
• determining the sex of the at least one yellowtail based on the result of the identifying step, and
• separating the at least one yellowtail into male or female based on the result of the determining step and culturing the male and female yellowtails separately,
• wherein the at least one yellowtail is at least one selected from the group consisting of Japanese amberjack, Greater amberjack and Yellowtail amberjack,
• wherein the exon 3 of the HSD17B1 gene of Japanese amberjack is set forth in SEQ ID NO:
• 3,
• wherein the exon 3 of the HSD17B1 gene of Greater amberjack is set forth in SEQ ID NO:
• 6,
• wherein the exon 3 of the HSD17B1 gene of Yellowtail amberjack is set forth in SEQ ID NO:
• 9,
• wherein the sex of the yellowtail is determined to be male when the genotype at the 169th nucleotide residue in the exon 3 of the HSD17B1 gene is an A/A, and
• wherein the sex of yellowtail is determined to be female when the genotype at the 169th nucleotide residue in the exon 3 of the HSD17B1 gene is a G/G or an A/G.