Nucleic acid molecules encoding DNA modifying enzymes and active fragments and variants thereof and compositions comprising the same
AssigneeLife Edit Therapeutics, Inc.
InventorsTyson D. Bowen, Alexandra Briner Crawley, Tedd D. Elich
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Abstract
Compositions and methods comprising novel deaminase polypeptides for targeted editing of nucleic acids are provided. Compositions comprise deaminase polypeptides. Also provided are fusion proteins comprising a DNA-binding polypeptide and a deaminase of the invention. The fusion proteins include RNA-guided nucleases fused to deaminases, optionally in complex with guide RNAs. Compositions also include nucleic acid molecules encoding the deaminases or the fusion proteins. Vectors and host cells comprising the nucleic acid molecules encoding the deaminases or the fusion proteins are also provided.
Claims22
A nucleic acid molecule comprising a polynucleotide encoding a deaminase polypeptide, wherein the deaminase polypeptide has adenine deaminase activity and is encoded by a nucleotide sequence that
- a) has at least 90% sequence identity to SEQ ID NO:
- 451, and
- b) encodes an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 407,
- wherein said nucleic acid molecule further comprises a heterologous promoter operably linked to said polynucleotide.
A nucleic acid molecule comprising a polynucleotide encoding a deaminase polypeptide, wherein said deaminase polypeptide has adenine deaminase activity and is encoded by a nucleotide sequence that
- a) has the sequence of SEQ ID NO:
- 451, or
- b) encodes the amino acid sequence of SEQ ID NO:
- 407.
A nucleic acid molecule comprising a polynucleotide encoding a fusion protein, wherein said fusion protein comprises:
- a Type II CRISPR-Cas protein nickase and a deaminase,
- wherein said deaminase has adenine deaminase activity and comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 407, and wherein said nickase
- (a) is a Cas9 nickase;
- or
- (b) comprises an amino acid sequence having at least 95% sequence identity to any one of SEQ ID NO:
- 42, 52, 53, 55-59, 61, 397, or 398.
Dependencies are inferred from the claim text.