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US 12,188,055 B2Granted
Engineered glucosyltransferases
AssigneeNutrition & Biosciences USA 4, Inc.
InventorsYougen Li, Ellen D. Semke, Qiong Cheng, Jian Ping Lai
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Abstract
Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
Claims20
A polynucleotide comprising a nucleotide sequence encoding a non-native glucosyltransferase,
- wherein the non-native glucosyltransferase comprises at least two amino acid substitutions at positions corresponding with amino acid residues Gln-588, Phe-607, or Arg-741 of SEQ ID NO:62,
- wherein the non-native glucosyltransferase synthesizes insoluble alpha-glucan comprising 1,3-linkages,
- wherein the non-native glucosyltransferase has:
- (i) an insoluble alpha-glucan yield that is higher than the insoluble alpha-glucan yield of a second glucosyltransferase that only differs from the non-native glucosyltransferase at the substitution positions, and/or a leucrose yield that is lower than the leucrose yield of the second glucosyltransferase;
- wherein the non-native glucosyltransferase comprises an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of residues 55-960 of SEQ ID NO:4 or the amino acid sequence of residues 54-957 of SEQ ID NO:
- 65.
3 more elements
Dependencies are inferred from the claim text.