Modified monooxygenases for the manufacture of hydroxylated hydrocarbons based on substitution of amino acids by alanine

A modified P450 monooxygenase having an amino acid sequence with at least 95% sequence identity with SEQ ID NO.:

• 1, wherein at least one amino acid of SEQ ID NO.:
• 1 selected from the group consisting of leucine 87, glutamic acid 88, lysine 89, isoleucine 90, threonine 91, proline 92, valine 93, serine 94, glutamic acid 96, threonine 98, threonine 100, leucine 101, arginine 103, tyrosine 104, aspartic acid 105, histidine 196, threonine 197, valine 198, asparagine 199, threonine 200, tryptophan 201, glycine 202, arginine 203, proline 204, proline 206, glutamic acid 207, glutamic acid 208, glutamine 209, valine 210, and combinations thereof is substituted by alanine, leading to an improved reactivity on hydroxylation of aromatic hydrocarbons as compared to a non-modified P450 oxygenase.