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US 12,188,051 B2Granted
Compositions and methods for obtaining vascularized human intestinal organoid tissue, and related uses thereof
AssigneeThe Regents of the University of Michigan
InventorsEmily Holloway, Jason Spence
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Abstract
The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or organ(s) through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting vascularized human intestinal organoid tissue having an intestine-specific EC transcriptional signature from hindgut spheroid tissue produced in vitro from the described methods.
Claims9
A method, comprising:
- culturing hindgut spheroid tissue in vitro, wherein the culturing results in differentiation of the hindgut spheroid tissue into tissue comprising vascularized human intestinal organoid tissue,
- wherein the culturing of the hindgut spheroid tissue in vitro comprises the following four successive culturing steps:
- a) incubating the hindgut spheroid tissue with a media comprising EGF, Noggin and R-Spondin2 (ENR media), wherein EGF is epidermal growth factor;
- b) incubating the result of step a) with ENR media and vascular endothelial growth factor (VEGF);
- c) incubating the result of step b) with EGF, bone morphogenic protein 4 (BMP4) and basic fibroblast growth factor (bFGF);
- and
- d) incubating the result of step c with EGF and VEGF.
4 more elements
Dependencies are inferred from the claim text.