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US 12,188,026 B2Granted
Surface expression vector using poly-gamma-glutamate synthetic gene derived from strain in Bacillus, and method for expressing protein on surface of microorganism, using same
AssigneeBioleaders Corporation
InventorsYoung Chul Park, Jae Won Jeon, Hong Gyu Park, Hyun Jun Kang, Se Eun Byeon, Kwang Seo Park, Dae Eun Ki
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Abstract
Provided is a surface expression vector having pgsA, a gene encoding poly-gamma-glutamate synthetase, and a method of expressing a target protein on the microbial surface using the vector. The vector having foreign genes inserted therein is transformed into a microorganism and allows a foreign protein to be stably expressed on the surface of the microorganism.
Claims14
A surface expression vector for expression of a target protein, the surface expression vector comprising:
- a gene encoding pgsA;
- and
- a gene encoding a target protein,
- wherein the gene encoding the pgsA consists of the nucleotide sequence of any one of SEQ ID NOs:
- 13 to 15 and 24 to 26.
2 more elements
An expression vector, comprising:
- an aldolase promoter;
- a pgsA gene from a Bacillus sp. encoding a poly-gamma-glutamate synthetase complex surface-anchoring motif consisting of the nucleotide sequence of any one of SEQ ID Nos:
- 13-15 and 24-26;
- a linker at a terminal end of the pgsA gene sequence;
- and
- a gene encoding a target protein.
3 more elements
Dependencies are inferred from the claim text.