Genus Hydrogenophilus bacterium transformant
AssigneesUtilization of Carbon Dioxide Institute Co., Ltd., The University of Tokyo
InventorsHideaki Yukawa, Naoto Ohtani, Masaharu Ishii
Life of this patent
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Abstract
A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source. (a1) DNA which consists of a base sequence of SEQ ID NO: 1; (a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity; (a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.
Claims21
A transformant obtained by introducing a pyruvate decarboxylase gene of (c1), (c2), (c4), (c5), or (c6) below, and an alcohol dehydrogenase gene, into a bacterium of genus Hydrogenophilus:
- (c1) DNA which comprises a base sequence of SEQ ID NO:
- 10;
- (c2) DNA which comprises a base sequence having 90% or more identity with SEQ ID NO:
- 10, the DNA encoding a polypeptide having pyruvate decarboxylase activity;
- (c4) DNA which encodes a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 11;
- (c5) DNA which encodes a polypeptide comprising an amino acid sequence having 90% or more identity with SEQ ID NO:
- 11, the polypeptide having pyruvate decarboxylase activity;
- (c6) DNA which encodes a polypeptide comprising an amino acid sequence having a deletion, substitution, or addition of one to five of amino acids in the amino acid sequence of SEQ ID NO:
- 11, the polypeptide having pyruvate decarboxylase activity.
Dependencies are inferred from the claim text.