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US 12,186,757 B2Granted
Devices and methods for monitoring and quantifying nucleic acid amplification
AssigneeThe Trustees of the University of Pennsylvania
InventorsChangchun Liu, Haim H. Bau
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Abstract
Provided herein are devices for monitoring nucleic acid amplification, nucleic acid amplification monitors, methods of quantifying nucleic acid amplification, methods of identifying an unknown nucleic acid molecule, and systems for monitoring nucleic acid amplification. The disclosed devices, methods, and systems comprise at least one nuclemeter, comprising at least one sample chamber and at least one reaction-diffusion conduit in fluid communication with the chamber.
Claims20
A device for monitoring nucleic acid amplification, the device comprising:
- at least one nuclemeter, comprising:
- (a) a sample chamber;
- (b) one or more reaction-diffusion conduits, the one or more reaction-diffusion conduits being in fluid communication with the sample chamber;
- wherein the sample chamber, the one or more reaction-diffusion conduits, or the sample chamber and the one or more reaction-diffusion conduits contain a polymer comprising one or more amplification reagents;
- (c) a removable barrier located between the sample chamber and each of the one or more reaction-diffusion conduits, wherein the removable barrier comprises a material that degrades, dissolves, melts, or any combination thereof;
- and
- (d) a measuring device configured to measure a reaction-diffusion length along the one or more reaction-diffusion conduits.
4 more elements
Dependencies are inferred from the claim text.