Time-resolved fluorescence immunochromatographic kit for simultaneous detection of mixed contamination of five mycotoxins such as aflatoxin B1 and application thereof

An immunochromatographic time-resolved fluorescence kit for simultaneously detecting mixed pollution of aflatoxin B1, fumonisin B1, ochratoxin A, zearalenone and sterigmatocystin, comprising:

• an immunochromatographic time-resolved fluorescence test strip and a sample reaction bulb containing an europium-labeled aflatoxin B1 monoclonal antibody freeze-dried product, an europium-labeled fumonisin B1 monoclonal antibody freeze-dried product, an europium-labeled ochratoxin A monoclonal antibody freeze-dried product, an europium-labeled zearalenone monoclonal antibody freeze-dried product and an europium-labeled sterigmatocystin monoclonal antibody freeze-dried product, wherein the immunochromatographic time-resolved fluorescence test strip comprises a PVC substrate, an absorbent pad, a test pad and a sample pad sequentially stuck to one face of the substrate from top to bottom, all the adjacent pads are in overlapped connection at joints, the test pad employs a cellulose nitrate film as a base pad, a transverse quality control line and 5 test lines are arranged on the cellulose nitrate film from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the 5 test lines are separately coated with an aflatoxin B1-bovine serum albumin conjugate, a fumonisin B1-bovine serum albumin conjugate, an ochratoxin A-bovine serum albumin conjugate, a zearalenone-bovine serum albumin conjugate and a sterigmatocystin-bovine serum albumin conjugate, and wherein
• said aflatoxin B1 monoclonal antibody is secreted from hybridoma cell line 3G1 that is deposited with an accession number of CCTCC NO. C201014;
• said fumonisin B1 monoclonal antibody is secreted from a hybridoma cell line Fm7A11that is deposited with an accession number of CCTCC NO. C201636;
• said ochratoxin A monoclonal antibody is secreted from hybridoma cell line 1H2 that is deposited with an accession number of CCTCC NO. C201329;
• said zearalenone monoclonal antibody is secreted from hybridoma cell line 2D3 that is deposited with accession number of CCTCC NO. C201328;
• and
• said sterigmatocystin monoclonal antibody is secreted from hybridoma cell line ST03that is deposited with an accession number of CCTCC NO. C2013187.