# US 12,181,468 B2 · Rotors for immunoassays > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,181,468 B2 - Document ID: US12181468B2 - Status: Granted - Kind: B2 - Application number: 17/195,118 - Filed: 2021-03-08 - Published: 2021-07-01 - Granted: 2024-12-31 - Earliest claimed: 2010-06-17 - Assignees: Zoetis Services LLC - Applicants: Zoetis Services LLC - Inventors: Rajesh K. Mehra; Kenneth P. Aron - Web page: https://patentgrep.com/patent/US12181468B2 ## CPC - G01N 33/54366: {Apparatus specially adapted for solid-phase testing} - B01L 3/5023: {with a sample being transported to, and subsequently stored in an absorbent for analysis} - G01N 33/54389: {with bidirectional or multidirectional lateral flow, e.g. wherein the sample flows from a single, common sample application point into multiple strips, lanes or zones} - G01N 33/581: {with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)} - G01N 33/587: {Nanoparticles} - B01L 2300/0681: Filter - B01L 2300/069: Absorbents; Gels to retain a fluid - B01L 2300/0806: Standardised forms, e.g. compact disc [CD] format - B01L 2300/0819: Microarrays; Biochips - B01L 2300/0864: comprising only one inlet and multiple receiving wells, e.g. for separation, splitting - B01L 2400/0409: centrifugal forces - G01N 35/00069: {whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract The present invention provides an analyte detection system for detecting target analytes in a sample. In particular, the invention provides a detection system in a rotor or disc format that utilizes a centrifugal force to move the sample through the detection system. Methods of using the rotor detection system to detect analytes in samples, particularly biological samples, and kits comprising the rotor detection system are also disclosed. ## Claims ### Claim 1 A device comprising a sample port and a surface, wherein the surface comprises a single channel in fluid communication with the sample port and a circular flow path at a peripheral edge of the surface, wherein the sample port and/or the channel comprises a conjugate comprising a binding partner that can specifically bind to an analyte, and wherein the circular flow path comprises an immobilized capture ligand capable of binding an analyte-conjugate complex. ### Claim 2 (depends on 1) The device of claim 1, wherein spinning the surface and the sample port about a fixed axis generates a centrifugal force that causes a sample deposited in the sample port to flow through the channel and come into fluid contact with the capture ligand. ### Claim 3 (depends on 1) The device of claim 1, wherein the sample port and/or channel comprises a plurality of conjugates. ### Claim 4 (depends on 1) The device of claim 1, wherein the circular flow path comprises a plurality of capture ligands. ### Claim 5 (depends on 1) The device of claim 1, wherein the binding partner is conjugated to a detectable entity. ### Claim 6 (depends on 5) The device of claim 5, wherein the detectable entity is a metallic nanoparticle or metallic nanoshell. ### Claim 7 (depends on 5) The device of claim 5, wherein the detectable entity is selected from the group consisting of gold nanoparticles, silver nanoparticles, copper nanoparticles, platinum nanoparticles, cadmium nanoparticles, composite nanoparticles, gold hollow spheres, gold-coated silica nanoshells, and silica-coated gold shells. ### Claim 8 (depends on 5) The device of claim 5, wherein the detectable entity is an enzyme. ### Claim 9 (depends on 8) The device of claim 8, wherein the enzyme is selected from the group consisting of alkaline phosphatase, horseradish peroxidase, beta-galactosidase, beta-lactamase, luciferase, myeloperoxidase, and amylase. ### Claim 10 (depends on 1) The device of claim 1, wherein the surface further comprises at least one absorbing entity located downstream from the capture ligand. ### Claim 11 (depends on 3) The device of claim 3, wherein the plurality of conjugates comprises at least a first conjugate capable of binding a first analyte and a second conjugate capable of binding a second analyte, wherein the first and second analytes are different. ### Claim 12 (depends on 3) The device of claim 3, wherein each conjugate in the plurality of conjugates is capable of binding a different analyte. ### Claim 13 (depends on 3) The device of claim 3, wherein the plurality of conjugates comprise binding partners conjugated to different detectable entities. ### Claim 14 (depends on 3) The device of claim 3, wherein each of said plurality of immobilized capture ligands specifically binds to a different analyte-conjugate complex. ### Claim 15 (depends on 3) The device of claim 3, wherein each of said plurality of immobilized capture ligands are capable of specifically binding to the same analyte-conjugate complex. ### Claim 16 (depends on 15) The device of claim 15, wherein the immobilized capture ligands are immobilized at different concentrations. ### Claim 17 (depends on 4) The device of claim 4, wherein the plurality of capture ligands are arranged sequentially along the circular path. ### Claim 18 (depends on 1) The device of claim 1, wherein the sample port comprises a blood separator material that allows plasma from a blood sample to pass into the channel, while retaining cellular material from the blood sample in the sample port. ### Claim 19 (depends on 1) A system for detecting target analytes in a sample, comprising the device of claim 1.