# US 12,181,466 B2 · Controls for implementing multiplex analysis methods > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,181,466 B2 - Document ID: US12181466B2 - Status: Granted - Kind: B2 - Application number: 17/577,147 - Filed: 2022-01-17 - Published: 2022-05-05 - Granted: 2024-12-31 - Earliest claimed: 2014-04-04 - Assignees: Bio-Rad Europe GmbH - Applicants: Bio-Rad Europe GmbH - Inventors: Christophe Rene Roger Vedrine; Nadine Marie Renee Lambert - Web page: https://patentgrep.com/patent/US12181466B2 ## CPC - G01N 33/54306: {Solid-phase reaction mechanisms} - G01N 33/54326: {Magnetic particles} - G01N 33/54366: {Apparatus specially adapted for solid-phase testing} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The present invention relates to controls which may be used to secure the results of multiplex analysis methods. The present invention thus relates to solid supports comprising one or several controls and their use in multiplex analysis methods to detect several analytes potentially present in a sample. ## Claims ### Claim 1 A multiplex analysis method for detecting at least n analytes in at least one sample, n being an integer greater than or equal to 2, said method comprising at least steps a), c) and e) or at least steps a), b), c) and d) as follows: a) providing at least one solid support, b) placing, in the presence of the spots of said compartment: l detection ligands of p analytes to be detected and at least one additive, l being greater than or equal to p, c) placing a sample to be analyzed in the presence of the spots of said compartment, d) placing, in the presence of the spots of said compartment: l′ detection ligands of m analytes to be detected, and, at least one detection ligand of said additive(s), l′ being greater than or equal to m, and e) placing, in the presence of the spots of said compartment: at least one detection ligand of a control compound and l″ detection ligands of y analytes to be detected, l″ being greater than or equal to y, l, l′, l″, m, p and y being integers greater than or equal to 0 and the sum m+p+y being greater than or equal to 1; said solid support comprising at least one compartment, said compartment comprising a plurality of spots, each spot being deposited at a separate location within said at least one compartment, said plurality of spots comprising two or more control spots deposited upon said solid support within said at least one compartment and at least two detection spots comprising a ligand specific for an analyte in a sample, and said at least two control spots being selected from the group consisting of a spot for controlling the deposition of a sample, a spot for controlling the deposition of an analyte detection ligand and a spot for controlling the deposition of a reporter, wherein: a) the spot for controlling the deposition of the sample comprises at least one control capture ligand specific for a control compound naturally present in the sample to be analyzed, said control capture ligand being an antibody, antigen, peptide, carbohydrate, lipid, or nucleic acid; b) the spot for controlling the deposition of the detection ligand for the analyte comprises at least one additive capture ligand specific for an additive that is added to a sample and said additive is not naturally present in the sample or derived from any compound present in the sample, said at least one additive capture ligand being an antibody, antigen, peptide, carbohydrate, lipid, or nucleic acid; c) the spot for controlling the deposition of the reporter comprises a direct marker, an indirect marker or a carrier molecule coupled to an indirect marker, wherein the reporter specifically interacts with the indirect marker or the indirect marker coupled to the carrier molecule, optionally, in presence of a substrate, to produce a detectable signal, said direct marker being selected from a radioisotope, a fluorochrome, a lanthanide, a luminescent compound, a transition metal, and colored, fluorescent or luminescent nanoparticles, said indirect marker being an enzyme, biotin, avidin, streptavidin, neutravidin, a hapten, an antigen or an antibody, said carrier being a polypeptide, functionalized polymer, a copolymer, or an antibody, and said reporter being an enzymatic substrate for an enzyme, an enzyme for a luminescent compound, and avidin, streptavidin, or neutravidin coupled to a direct or indirect marker for biotin, and said ligands for analyte detection are antibodies, antigens, peptides, carbohydrates, lipids, or nucleic acids. ### Claim 2 (depends on 1) The multiplex analysis method according to claim 1, comprising a subsequent step f) of placing at least one reporter in the presence of the spots of said compartment. ### Claim 3 (depends on 2) The multiplex analysis method according to claim 2, characterized in that the reporter or at least one of the reporters in step f) is coupled to an indirect marker, and in that said method comprises a subsequent step g) of placing at least one second reporter of said indirect marker coupled to said reporter of step f) in the presence of the spots of said compartment. ### Claim 4 (depends on 3) The multiplex analysis method according to claim 3, characterized in that said second reporter is a substrate. ### Claim 5 (depends on 4) The multiplex analysis method according to claim 4, wherein said substrate is luminol, isoluminol or a derivative thereof. ### Claim 6 A multiplex analysis method for detecting at least n analytes in at least one sample, n being an integer greater than or equal to 2, said method comprising at least steps a), c) and e) or at least steps a), b), c) and d) as follows: a) providing at least one solid support comprising at least one set of beads comprising at least one control bead and at least two detection beads for an analyte, characterized in that the control bead is selected from the group consisting of a bead for controlling the deposition of a sample, a bead for controlling the deposition of a detection ligand of an analyte and a bead for controlling the deposition of a reporter, b) placing, in the presence of said at least one set of beads: l detection ligands of p analytes to be detected and at least one additive, l being greater than or equal to p, c) placing a sample to be analyzed in the presence of said at least one set of beads, d) placing, in the presence of said at least one set of beads: l′ detection ligands of m analytes to be detected, and, at least one detection ligand of said additive(s), l′ being greater than or equal to m, and e) placing, in the presence of said at least one set of beads: at least one detection ligand of a control compound and l″ detection ligands of y analytes to be detected, l″ being greater than or equal to y, l, l′, l″, m, p and y being integers greater than or equal to 0 and the sum m+pty being greater than or equal to 1. ### Claim 7 (depends on 6) The multiplex analysis method according to claim 6, comprising a subsequent step f) of placing at least one reporter in the presence of said at least one set of beads. ### Claim 8 (depends on 7) The multiplex analysis method according to claim 7, characterized in that the reporter or at least one of the reporters in step f) is coupled to an indirect marker, and in that said method comprises a subsequent step g) of placing at least one second reporter of said indirect marker coupled to said reporter of step f) in the presence of the spots of said compartment or of said set of beads. ### Claim 9 (depends on 8) The multiplex analysis method according to claim 8, characterized in that said second reporter is a substrate. ### Claim 10 (depends on 9) The multiplex analysis method according to claim 9, wherein said substrate is luminol, isoluminol or a derivative thereof. ### Claim 11 (depends on 6) The multiplex analysis method according to claim 6, said at least one set of beads comprising at least one bead for controlling the deposition of a sample, at least one bead for controlling the deposition of a detection ligand of an analyte and optionally at least one bead for controlling the deposition of a reporter.