# US 12,181,463 B2 · Performing optical measurements on a sample > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,181,463 B2 - Document ID: US12181463B2 - Status: Granted - Kind: B2 - Application number: 17/360,503 - Filed: 2021-06-28 - Published: 2021-10-21 - Granted: 2024-12-31 - Earliest claimed: 2016-05-11 - Assignees: S.D. Sight Diagnostics Ltd. - Applicants: S.D. Sight Diagnostics Ltd. - Inventors: Amir Zait; Arnon Houri Yafin; Dan Gluck; Sharon Pecker; Yochay Shlomo Eshel; Sarah Levy Schreier; Joseph Joel Pollak - Web page: https://patentgrep.com/patent/US12181463B2 ## CPC - G01N 33/5094: {for blood cell populations (red blood cells G01N33/80)} - G01N 15/06: Investigating concentration of particle suspensions (by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10) - G01N 33/49: Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} - G01N 33/56972: {White blood cells} - G01N 33/721: {Haemoglobin} - G01N 2015/012: {Red blood cells} - G01N 2015/016: {White blood cells} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Apparatus and methods are described including measuring hematocrit within a blood sample, by performing a first measurement on a first portion of the blood sample. Mean corpuscular volume in the blood sample is measured, by performing a second measurement on a second portion of the blood sample, the second portion being diluted with respect to the first portion. A red blood cell count per unit volume within the blood sample is determined by dividing the hematocrit measured within the first portion by the mean corpuscular volume measured within the second portion. Other applications are also described. ## Claims ### Claim 1 A method for use with a blood sample, the method comprising: measuring hematocrit within the blood sample, by performing a first measurement on a first portion of the blood sample; measuring mean corpuscular volume in the blood sample, by performing a second measurement on a second portion of the blood sample, the second portion being diluted with respect to the first portion; determining a red blood cell count per unit volume within the blood sample, by dividing the hematocrit measured within the first portion by the mean corpuscular volume measured within the second portion; and determining counts of one or more components per unit volume within the blood sample, based on the red blood cell count per unit volume within the blood sample, by: analyzing a microscopic image of at least one of the first and second portions of the blood sample, such as to determine a count of the one or more components and count of red blood cells within the at least one of the first and second portions of the blood sample, determining a ratio between the count of the one or more components and the count of red blood cells within the at least one of the first and second portions of the blood sample, and determining the count of the one or more components per unit volume within the blood sample by multiplying the red blood cell count per unit volume by the ratio. ### Claim 2 (depends on 1) The method according to claim 1, wherein measuring hematocrit within the blood sample comprises performing a measurement on the first portion of the blood sample selected from the group consisting of: a micro-hematocrit-method measurement, an impedance measurement, and an ultrasonic measurement. ### Claim 3 (depends on 1) The method according to claim 1, wherein analyzing the microscopic image of at least one of the first and second portions of the blood sample comprises analyzing a microscopic image of the first portion of the blood sample. ### Claim 4 (depends on 1) The method according to claim 1, wherein analyzing the microscopic image of at least one of the first and second portions of the blood sample comprises analyzing a microscopic image of the second portion of the blood sample. ### Claim 5 (depends on 1) The method according to claim 1, wherein performing the second measurement on the second portion of the blood sample comprises performing microscopic bright-field imaging on the second portion of the blood sample. ### Claim 6 (depends on 1) The method according to claim 1, wherein the one or more components within the blood sample are selected from the group consisting of: red blood cells of a given type, white blood cells, white blood cells of a given type, circulating tumor cells, platelets, platelets of a given type, bacteria, pathogens, pathogens of a given type, reticulocytes, and Howell-Jolly bodies. ### Claim 7 Apparatus for use with a blood sample, the apparatus comprising: at least one computer processor configured to: measure hematocrit in the blood sample, by performing a first measurement on a first portion of the blood sample; measure mean corpuscular volume in the blood sample, by performing a second measurement on a second portion of the blood sample, the second portion being diluted with respect to the first portion; determine a red blood cell count per unit volume within the blood sample, by dividing the hematocrit measured within the first portion by the mean corpuscular volume measured within the second portion; and determine counts of one or more components per unit volume within the blood sample, based on the red blood cell count per unit volume within the blood sample, by: analyzing a microscopic image of at least one of the first and second portions of the blood sample, such as to determine a count of the one or more components and count of red blood cells within the at least one of the first and second portions of the blood sample, determining a ratio between the count of the one or more components and the count of red blood cells within the at least one of the first and second portions of the blood sample, and determining the count of the one or more components per unit volume within the blood sample by multiplying the red blood cell count per unit volume by the ratio. ### Claim 8 (depends on 7) The apparatus according to claim 7, wherein the at least one computer processor is configured to perform the first measurement on the first portion of the blood sample by performing a measurement on the first portion of the blood sample selected from the group consisting of: a micro-hematocrit-method measurement, an impedance measurement, and an ultrasonic measurement. ### Claim 9 (depends on 7) The apparatus according to claim 7, wherein the at least one computer processor is configured to analyze the microscopic image of at least one of the first and second portions of the blood sample by analyzing a microscopic image of the first portion of the blood sample. ### Claim 10 (depends on 7) The apparatus according to claim 7, wherein the at least one computer processor is configured to analyze the microscopic image of at least one of the first and second portions of the blood sample by analyzing a microscopic image of the second portion of the blood sample. ### Claim 11 (depends on 7) The apparatus according to claim 7, wherein the at least one computer processor is configured to perform the second measurement on the second portion of the blood sample by performing microscopic bright-field imaging on the second portion of the blood sample. ### Claim 12 (depends on 7) The apparatus according to claim 7, wherein the one or more components within the blood sample are selected from the group consisting of: red blood cells of a given type, white blood cells, white blood cells of a given type, circulating tumor cells, platelets, platelets of a given type, bacteria, pathogens, pathogens of a given type, reticulocytes, and Howell-Jolly bodies. ### Claim 13 A computer software product, for use with a blood sample, the computer software product comprising a non-transitory computer-readable medium in which program instructions are stored, which instructions, when read by a computer cause the computer to perform the steps of: measuring hematocrit within the blood sample, by performing a first measurement on a first portion of the blood sample; measuring mean corpuscular volume in the blood sample, by performing a second measurement on a second portion of the blood sample, the second portion being diluted with respect to the first portion; and determining a red blood cell count per unit volume within the blood sample, by dividing the hematocrit measured within the first portion by the mean corpuscular volume measured within the second portion; and determining counts of one or more components per unit volume within the blood sample, based on the red blood cell count per unit volume within the blood sample, by: analyzing a microscopic image of at least one of the first and second portions of the blood sample, such as to determine a count of the one or more components and count of red blood cells within the at least one of the first and second portions of the blood sample, determining a ratio between the count of the one or more components and the count of red blood cells within the at least one of the first and second portions of the blood sample, and determining the count of the one or more components per unit volume within the blood sample by multiplying the red blood cell count per unit volume by the ratio.