# US 12,181,453 B2 · Chromatography column positioning assemblies

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- Number: US 12,181,453 B2
- Document ID: US12181453B2
- Status: Granted
- Kind: B2
- Application number: 17/512,864
- Filed: 2021-10-28
- Published: 2022-04-28
- Granted: 2024-12-31
- Earliest claimed: 2020-10-28
- Assignees: Waters Technologies Corporation
- Applicants: Waters Technologies Corporation
- Inventors: Wade P. Leveille; Joseph D. Michienzi; Jeffrey Musacchio; Michael O. Fogwill
- Web page: https://patentgrep.com/patent/US12181453B2

## CPC

- G01N 30/6047: {with supporting means; Holders}
- B01D 15/22: relating to the construction of the column
- G01N 30/6004: {end pieces}
- G01N 30/6026: {Fluid seals}
- G01N 30/6052: {body}
- G01N 30/461: {with serial coupling of separation columns}
- G01N 30/6039: {in series}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)

## Abstract

The exemplary embodiments provide chromatography column positioning assemblies that can ensure that the distance between face seals or other sealing surfaces/mechanisms at the respective ends of a liquid chromatography column is a desired distance (i.e., the length of the liquid chromatography column). The exemplary embodiments can adjust the separation between the face seals to accommodate different length liquid chromatography columns. For example, a chromatography column positioning assembly of an exemplary embodiment can set the distance between face seals to accommodate a 25 mm column, a 50 mm column or a 100 mm column.

## Claims

### Claim 1

A chromatography column positioning assembly for accommodating different chromatography column lengths, comprising:
an inlet face seal;
an outlet face seal;
a chromatography column holder of a first length for holding a chromatography column in a position for creating a fluidic connection, the chromatography column holder:
being configured for multiple uses with chromatography columns of different lengths,
having a length to engage with the inlet face seal and the outlet face seal and which is a length of a longest chromatography column for which the chromatography column holder is configured for use with; and
an extender for positioning in the chromatography column holder to occupy at least a portion of the first length of the chromatography column holder that is not occupied when the chromatography column holder holds a chromatography column of a second length, where the second length is less than the first length.

### Claim 2 (depends on 1)

The chromatography column positioning assembly of claim 1, wherein the extender has a length substantially equal to the first length minus the second length.

### Claim 3 (depends on 1)

The chromatography column positioning assembly of claim 1, further comprising an additional extender of a third length that differs from the second length.

### Claim 4 (depends on 1)

The chromatography column positioning assembly of claim 1, wherein the chromatography column holder includes an inner lumen configured to hold the chromatography column.

### Claim 5 (depends on 4)

The chromatography column positioning assembly of claim 4, wherein the extender is configured to be positioned in the inner lumen of the chromatography column holder.

### Claim 6 (depends on 1)

The chromatography column positioning assembly of claim 1, wherein the extender is integrated with the column.

### Claim 7 (depends on 6)

The chromatography column positioning assembly of claim 6, wherein the extender is configured so that the inner lumen of the extender is aligned with an inner lumen of the chromatography column when the extender is held by the chromatography column holder to create a continuous flow path through the chromatography column holder.

### Claim 8 (depends on 1)

The chromatography column positioning assembly of claim 1, wherein the extender and the column are fully enveloped by the chromatography column holder when held by the chromatography column holder.

### Claim 9 (depends on 1)

The chromatography column positioning assembly of claim 1, wherein at least one of the extender and the column is only partially enveloped by the chromatography column holder when held by the chromatography column holder.