# US 12,181,434 B2 · Biochemical test chip

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- Number: US 12,181,434 B2
- Document ID: US12181434B2
- Status: Granted
- Kind: B2
- Application number: 17/339,599
- Filed: 2021-06-04
- Published: 2022-05-26
- Granted: 2024-12-31
- Earliest claimed: 2020-11-25
- Assignees: Apex Biotechnology Corp.
- Applicants: Apex Biotechnology Corp.
- Inventors: Chen-Yu Yang; Cheng-Yu Chou
- Web page: https://patentgrep.com/patent/US12181434B2

## CPC

- G01N 27/3272: {Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52)}
- C12Q 1/001: {Enzyme electrodes}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present disclosure provides a biochemical test chip, including an insulating substrate, an electrode unit, a first insulating septum, a reactive layer and a second insulating septum. The electrode unit is located on the insulating substrate. The electrode unit includes a working electrode and a counter electrode. A current density of the counter electrode is greater than a current density of the working electrode. The first insulating septum is located on the electrode unit. The first insulating septum has an opening, which at least partially exposes the electrode unit. The reactive layer is located in the opening and is electrically connected to the electrode unit. The second insulating septum is located on the first insulating septum.

## Claims

### Claim 1

A biochemical test chip, comprising:
an insulating substrate;
an electrode unit, located on the insulating substrate, wherein the electrode unit comprises a working electrode and a counter electrode, wherein a current density of the counter electrode is greater than a current density of the working electrode;
a first insulating septum, located on the electrode unit, wherein the first insulating septum has an opening, and the opening at least partially exposes the electrode unit;
a reactive layer, located at the opening and electrically connected to the electrode unit; and
a second insulating septum, located on the first insulating septum;
wherein the reactive layer and a target analyte undergo a primary reaction, and the counter electrode is configured to undergo a self-redox reaction, wherein the self-redox reaction does not interfere with the primary reaction, and the self-redox reaction allows the counter electrode to have the capability of receiving or releasing additional electrons;
wherein the counter electrode is a cathode, and a standard reduction potential of an active material of the counter electrode satisfies Es0>Em0−Ev, where the Es0 is the standard reduction potential of the active material, the Em0 is a standard reduction potential for concentration reaction on the working electrode, and the Ev is a potential applied by a measuring apparatus when providing measuring reaction.

### Claim 2 (depends on 1)

The biochemical test chip of claim 1, wherein the current density of the counter electrode is greater than or equal to twice of the current density of the working electrode.

### Claim 3 (depends on 1)

The biochemical test chip of claim 1, wherein an area of the counter electrode is smaller than or equal to an area of the working electrode.

### Claim 4 (depends on 1)

The biochemical test chip of claim 1, wherein the counter electrode comprises a first portion and a second portion, wherein the first portion and the reactive layer do not overlap with each other.

### Claim 5 (depends on 1)

The biochemical test chip of claim 1, wherein the counter electrode comprises a first portion and a second portion, and the opening at least partially exposes the first portion.

### Claim 6 (depends on 1)

The biochemical test chip of claim 1, further comprising a protective layer, electrically connected to the electrode unit.

### Claim 7 (depends on 1)

The biochemical test chip of claim 1, wherein the electrode unit further comprises a second counter electrode, wherein the counter electrode and the second counter electrode are separated from each other.

### Claim 8 (depends on 7)

The biochemical test chip of claim 7, wherein a standard reduction potential of the counter electrode is greater than a standard reduction potential of the second counter electrode.

### Claim 9 (depends on 7)

The biochemical test chip of claim 7, wherein a sum of an area of the counter electrode and an area of the second counter electrode is smaller than or equal to an area of the working electrode.