# US 12,181,420 B2 · Z-axis measurement fixture and method of determining the planarity of objects using the fixture > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,181,420 B2 - Document ID: US12181420B2 - Status: Granted - Kind: B2 - Application number: 18/384,361 - Filed: 2023-10-26 - Published: 2024-03-07 - Granted: 2024-12-31 - Earliest claimed: 2020-09-30 - Assignees: IDEXX Laboratories, Inc. - Applicants: IDEXX Laboratories, Inc. - Inventors: David C. Giroux; Nathanael Williams - Web page: https://patentgrep.com/patent/US12181420B2 ## CPC - G01B 11/306: {for measuring evenness} - G01N 21/956: Inspecting patterns on the surface of objects {(contactless testing of electronic circuits G01R31/308; testing currency G07D; manufacturing processes per se of semiconductor devices implementing a measuring step H10P74/20)} - G01B 21/047: {Accessories, e.g. for positioning, for tool-setting, for measuring probes} - G01N 35/00623: {of instruments} - G01N 2035/00138: {Slides} - G01N 2035/00673: {of reagents} - G01B: MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A method of measuring the degree of Z-axis variability in a chemical reagent test slide, which includes the steps of placing a Z-axis measurement fixture on a surface of a gauge block of an optical measurement system having a camera, mounting the chemical reagent test slide on the Z-axis measurement fixture, and positioning the chemical reagent test slide mounted on the Z-axis measurement fixture in optical communication with the camera. ## Claims ### Claim 1 A method of measuring the degree of Z-axis variability or surface topography irregularities in a chemical reagent test slide, the chemical reagent test slide having an upper surface and a lower surface situated opposite the upper surface, the method comprising the steps of: placing a Z-axis measurement fixture on a surface of a gauge block of a measurement instrument, the Z-axis measurement fixture having a main body and a plurality of test slide supporting members, the main body having a top wall and a bottom wall disposed opposite the top wall, the main body defining a plurality of member receiving openings extending between the top wall and the bottom wall, each test slide supporting member of the plurality of test slide supporting members having a known and calibrated dimension in at least one coordinate direction, each test slide supporting member of the plurality of test slide supporting members being received by a respective member receiving opening of the plurality of member receiving openings extending in the main body, each test slide supporting member of the plurality of test slide supporting members having a first portion which projects outwardly from the top wall of the main body and a second portion which projects outwardly from or is at least level with the bottom wall of the main body, each test slide supporting member of the plurality of the test slide supporting members being arranged within the respective member receiving opening of the plurality of member receiving openings such that the first portion thereof projecting outwardly from the top wall of the main body and the second portion thereof projecting outwardly from or being level with the bottom wall of the main body are in alignment with the known and calibrated dimension of the test slide supporting member in the at least one coordinate direction, wherein the second portion of each test slide supporting member of the plurality of test slide supporting members engages the surface of the gauge block of the measurement instrument; mounting the chemical reagent test slide on the Z-axis measurement fixture such that the chemical reagent test slide rests on and is supported by the first portion of each test slide supporting member of the plurality of test slide supporting members; positioning the chemical reagent test slide mounted on the Z-axis measurement fixture on the measurement instrument; and determining by the measurement instrument the degree of Z-axis variability or surface topography irregularities in the chemical reagent test slide. ### Claim 2 A method of measuring the degree of Z-axis variability or surface topography irregularities in a chemical reagent test slide, the chemical reagent test slide having an upper surface and a lower surface situated opposite the upper surface, the method comprising the steps of: placing a Z-axis measurement fixture on a surface of a gauge block of an optical measurement instrument, the Z-axis measurement fixture having a main body and a plurality of test slide supporting members, the main body having a top wall and a bottom wall disposed opposite the top wall, the main body defining a plurality of member receiving openings extending between the top wall and the bottom wall, each test slide supporting member of the plurality of test slide supporting members having a known and calibrated dimension in at least one coordinate direction, each test slide supporting member of the plurality of test slide supporting members being received by a respective member receiving opening of the plurality of member receiving openings extending in the main body, each test slide supporting member of the plurality of test slide supporting members having a first portion which projects outwardly from the top wall of the main body and a second portion which projects outwardly from or is at least level with the bottom wall of the main body, each test slide supporting member of the plurality of the test slide supporting members being arranged within the respective member receiving opening of the plurality of member receiving openings such that the first portion thereof projecting outwardly from the top wall of the main body and the second portion thereof projecting outwardly from or being level with the bottom wall of the main body are in alignment with the known and calibrated dimension of the test slide supporting member in the at least one coordinate direction, wherein the second portion of each test slide supporting member of the plurality of test slide supporting members engages the surface of the gauge block of the optical measurement instrument, the optical measurement instrument further having a camera; mounting the chemical reagent test slide on the Z-axis measurement fixture such that the chemical reagent test slide rests on and is supported by the first portion of each test slide supporting member of the plurality of test slide supporting members; and positioning the chemical reagent test slide mounted on the Z-axis measurement fixture in optical communication with the camera of the optical measurement instrument. ### Claim 3 (depends on 2) A method as defined by claim 2, which further comprises the step of: imaging the chemical reagent test slide using the camera of the optical measurement instrument having a predetermined field of view at a first focal point or first focal area on an imaged surface of the chemical reagent test slide to provide a first optical image of the chemical reagent test slide in which the first focal point or first focal area on the imaged surface of the chemical reagent test slide is in focus in the first optical image, the first optical image being in a first X-Y plane in which the first focal point or first focal area, in focus in the first optical image, resides, the first X-Y plane establishing a zero reference plane for measuring the degree of Z-axis variability or surface topography irregularities in the chemical reagent test slide. ### Claim 4 (depends on 3) A method as defined by claim 3, which further comprises the step of: imaging the chemical reagent test slide using the camera of the optical measurement instrument at at least a second focal point or second focal area on the imaged surface of the chemical reagent test slide which is spaced from the first focal point or first focal area to provide at least a second optical image of the chemical reagent test slide in which the at least second focal point or second focal area is in focus in the at least second optical image, the at least second optical image being in an at least second X-Y plane in which the at least second focal point or second focal area, in focus in the at least second optical image, resides. ### Claim 5 (depends on 4) A method as defined by claim 4, which further comprises the step of: measuring the relative distance in the Z-axis between the zero reference plane and the at least second X-Y plane to provide a measured relative distance. ### Claim 6 (depends on 5) A method as defined by claim 5, which further comprises the step of: determining from the measured relative distance the degree of Z-axis variability or surface topography irregularities in the chemical reagent test slide. ### Claim 7 (depends on 2) A method as defined by claim 2, wherein each test slide supporting member of the plurality of test slide supporting members of the Z-axis measurement fixture is a spherical member having a known and calibrated diameter. ### Claim 8 (depends on 2) A method as defined by claim 2, wherein each test slide supporting member of the plurality of test slide supporting members of the Z-axis measurement fixture is a stainless steel ball having a known and calibrated diameter. ### Claim 9 (depends on 2) A method as defined by claim 2, wherein the plurality of test slide supporting members of the Z-axis measurement fixture includes three test slide supporting members spaced apart from each other. ### Claim 10 (depends on 2) A method as defined by claim 2, wherein the main body of the Z-axis measurement fixture includes at least one test slide edge guide projection extending outwardly from the top wall thereof, the at least one test slide edge guide projection being provided to help locate the chemical reagent test slide in a proper position above the top wall of the main body. ### Claim 11 (depends on 10) A method as defined by claim 10, wherein the at least one test slide edge guide projection includes a first generally V-shaped wedge projection and a second generally V-shaped wedge projection, the first wedge projection being spaced apart from the second wedge projection on the top wall of the main body; and wherein the chemical reagent test slide is receivable between the first and second wedge projections. ### Claim 12 (depends on 10) A method as defined by claim 10, wherein the at least one test slide edge guide projection includes a first generally V-shaped wedge projection, a second generally V-shaped wedge projection and a third projection, the first wedge projection being spaced apart from the second wedge projection on the top wall of the main body, the third projection being spaced apart from the first wedge projection and the second wedge projection on the top wall of the main body; and wherein the chemical reagent test slide is receivable between the first and second wedge projections and the third projection. ### Claim 13 A method of measuring the degree of Z-axis variability or surface topography irregularities in an object to be tested, the object to be tested having an upper surface and a lower surface situated opposite the upper surface, the method comprising the steps of: placing a Z-axis measurement fixture on a surface of a gauge block of an optical measurement instrument, the Z-axis measurement fixture having a main body and a plurality of object supporting members, the main body having a top wall and a bottom wall disposed opposite the top wall, the main body defining a plurality of member receiving openings extending between the top wall and the bottom wall, each object supporting member of the plurality of object supporting members having a known and calibrated dimension in at least one coordinate direction, each object supporting member of the plurality of object supporting members being received by a respective member receiving opening of the plurality of member receiving openings extending in the main body, each object supporting member of the plurality of object supporting members having a first portion which projects outwardly from the top wall of the main body and a second portion which projects outwardly from or is at least level with the bottom wall of the main body, each object supporting member of the plurality of object supporting members being arranged within the respective member receiving opening of the plurality of member receiving openings such that the first portion thereof projecting outwardly from the top wall of the main body and the second portion thereof projecting outwardly from or being level with the bottom wall of the main body are in alignment with the known and calibrated dimension of the object supporting member in the at least one coordinate direction, wherein the second portion of each object supporting member of the plurality of the object supporting members engages the surface of the gauge block of the optical measurement instrument, the optical measurement instrument further having a camera; mounting the object to be tested on the Z-axis measurement fixture such that the object to be tested rests on and is supported by the first portion of each object supporting member of the plurality of object supporting members; positioning the object to be tested mounted on the Z-axis measurement fixture in optical communication with the camera of the optical measurement instrument; imaging the object to be tested using the camera of the optical measurement instrument having a predetermined field of view at a first focal point or first focal area on an imaged surface of the object to be tested to provide a first optical image of the object in which the first focal point or first focal area on the imaged surface of the object is in focus in the first optical image, the first optical image being in a first X-Y plane in which the first focal point or first focal area, in focus in the first optical image, resides, the first X-Y plane establishing a zero reference plane for measuring the degree of Z-axis variability or surface topography irregularities in the object; successively imaging the object to be tested using the camera of the optical measurement instrument at successive spaced apart focal points or focal areas on the imaged surface of the object to provide successive optical images of the object in which the successive focal points or focal areas are respectively in focus, the successive optical images being in optical X-Y planes in which the respective focal points or focal areas reside and are in focus. ### Claim 14 (depends on 13) A method as defined by claim 13, which further comprises the step of: determining whether one or more of the optical X-Y planes are above the zero reference plane relative to the Z-axis and whether one or more of the optical X-Y planes is below the zero reference plane relative to the Z-axis. ### Claim 15 (depends on 14) A method as defined by claim 14, which further comprises the steps of: measuring the relative distances in the Z-axis between the zero reference plane and each successive optical X-Y plane to provide a plurality of measured relative distances; wherein, when all of the successive optical X-Y planes are situated above the zero reference plane, determining which of the measured relative distances is the greatest relative distance above the zero reference plane and determining the degree of Z-axis variability or surface topography irregularities in the object from the greatest relative distance above the zero reference plane; wherein, when all of the successive optical X-Y planes are situated below the zero reference plane, determining which of the measured relative distances is the greatest relative distance below the zero reference plane and determining the degree of Z-axis variability or surface topography irregularities in the object from the greatest relative distance below the zero reference plane; and wherein, when one or more of the successive optical X-Y planes are situated above the zero reference plane and one or more of the successive optical X-Y planes are situated below the zero reference plane, determining which of the measured relative distances for the successive optical X-Y planes above the zero reference plane is the greatest measured distance above the zero reference plane and determining which of the measured relative distances for the successive optical X-Y planes below the zero reference plane is the greatest measured distance below the zero reference plane and determining the degree of Z-axis variability or surface topography irregularities in the object from the greatest measured distance above the zero reference plane and the greatest measured distance below the zero reference plane. ### Claim 16 (depends on 13) A method as defined by claim 13, wherein each object supporting member of the plurality of object supporting members of the Z-axis measurement fixture is a spherical member having a known and calibrated diameter. ### Claim 17 (depends on 13) A method as defined by claim 13, wherein each object supporting member of the plurality of object supporting members of the Z-axis measurement fixture is a stainless steel ball having a known and calibrated diameter. ### Claim 18 (depends on 13) A method as defined by claim 13, wherein the plurality of object supporting members of the Z-axis measurement fixture includes three object supporting members spaced apart from each other. ### Claim 19 (depends on 13) A method as defined by claim 13, wherein the main body of the Z-axis measurement fixture includes at least one object edge guide projection extending outwardly from the top wall thereof, the at least one object edge guide projection being provided to help locate the object to be tested in a proper position above the top wall of the main body. ### Claim 20 (depends on 19) A method as defined by claim 19, wherein the at least one object edge guide projection includes a first generally V-shaped wedge projection and a second generally V-shaped wedge projection, the first wedge projection being spaced apart from the second wedge projection on the top wall of the main body; and wherein the object to be tested is receivable between the first and second wedge projections. ### Claim 21 (depends on 19) A method as defined by claim 19, wherein the at least one object edge guide projection includes a first generally V-shaped wedge projection, a second generally V-shaped wedge projection and a third projection, the first wedge projection being spaced apart from the second wedge projection on the top wall of the main body, the third projection being spaced apart from the first wedge projection and the second wedge projection on the top wall of the main body; and wherein the object to be tested is receivable between the first and second wedge projections and the third projection.