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US 12,181,409 B2Granted
Cell imaging composition and cellular material imaging method using same
AssigneesEwha University-Industry Collaboration Foundation, Kytecbio Co., Ltd.
InventorsKwan Mook Kim, Juyoung Yoon, Sun Shin Cha, Ah-Young Song
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Abstract
The present disclosure relates to a composition for cell imaging including a probe compound, and a method of cell-material imaging using the same. The composition for cell imaging and the method of cell-material imaging are provided to image the material in the cell by measuring fluorescence generated by reacting the probe and a material in a cell.
Claims16
A composition for cell imaging, comprising a probe compound represented by the following Chemical Formula 1:
- wherein,
- R1 is selected from the group consisting of hydrogen, a halogen group, an amino group, a C1-10 alkyl group, a C6-10 aryl group and a C1-10 alkoxy group,
- each of R3 and R4 is independently selected from the group consisting of hydrogen, a halogen group, a C1-10 alkyl group, a C6-10 aryl group and a C1-10 alkoxy group,
- each of R2 and R5 is independently selected from the group consisting of hydrogen, a halogen group, an amino group, a nitro group, a cyano group, a formyl group, a carboxyl group, a C1-10 alkyl group, a C1-10 alkylcarbonyl group, a C6-10 aryl group and a C1-10 alkoxy group,
- when the C1-10 alkyl group, the C1-10 alkylcarbonyl group, the C6-10 aryl group or the C1-10 alkoxy group is substituted, it is substituted with at least one substituent selected from the group consisting of a halogen group, a hydroxy group, an amino group, a cyano group, a nitro group and a C6-10 aryl group,
- Z is ═N— or ═CH—, and
- n is an integer of from 0 to 5.
4 more elements
A method of cell-material imaging, comprising:
- measuring fluorescence generated by reacting a probe compound represented by the following Chemical Formula 1, and a material in a target cell:
- wherein,
- R1 is selected from the group consisting of hydrogen, a halogen group, an amino group, a C1-10 alkyl group, a C6-10 aryl group and a C1-10 alkoxy group,
- each of R3 and R4 is independently selected from the group consisting of hydrogen, a halogen group, a C1-10 alkyl group, a C6-10 aryl group and a C1-10 alkoxy group,
- each of R2 and R5 is independently selected from the group consisting of hydrogen, a halogen group, an amino group, a nitro group, a cyano group, a formyl group, a carboxyl group, a C1-10 alkyl group, a C1-10 alkylcarbonyl group, a C6-10 aryl group and a C1-10 alkoxy group,
- when the C1-10 alkyl group, the C1-10 alkylcarbonyl group, the C6-10 aryl group or the C1-10 alkoxy group is substituted, it is substituted with at least one substituent selected from the group consisting of a halogen group, a hydroxy group, an amino group, a cyano group, a nitro group and a C6-10 aryl group,
- Z is ═N— or ═CH—, and
- n is an integer of from 0 to 5.
5 more elements
Dependencies are inferred from the claim text.