# US 12,181,399 B2 · Light-scattering detection device > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,181,399 B2 - Document ID: US12181399B2 - Status: Granted - Kind: B2 - Application number: 17/264,586 - Filed: 2019-07-05 - Published: 2022-02-10 - Granted: 2024-12-31 - Earliest claimed: 2018-08-01 - Assignees: Shimadzu Corporation - Applicants: Shimadzu Corporation - Inventors: Toru Yamaguchi; Atsushi Kasatani - Web page: https://patentgrep.com/patent/US12181399B2 ## CPC - G01N 15/0227: using imaging; using holography - G01N 15/0211: {Investigating a scatter or diffraction pattern} - G01N 30/74: Optical detectors {(measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J)} - G01N 21/53: within a flowing fluid, e.g. smoke - G01N 2015/0053: {in liquids, e.g. trouble} - G01N 2030/8831: {involving peptides or proteins} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A light-scattering detection device includes a transparent sample cell, a light source for emitting coherent light onto the sample cell, an imaging optics for collecting scattered light from the sample cell to the periphery thereof at different scattering angles, a slit plate for limiting the scattering angle range, a detector for receiving collected light from the imaging optics, and an aperture plate for limiting, by the aperture width thereof, the width of the light received by the detector, the aperture plate being arranged in the detector with respect to the focal length of the imaging optics. A plurality of detection optics is arranged around the sample cell at equal intervals from the central axis of the sample cell. The aperture width of each aperture plate differs in accordance with the arrangement angle θ of each detector with respect to the sample cell. ## Claims ### Claim 1 A light-scattering detection device for detecting fine particles in a liquid sample, comprising: a transparent sample cell configured to hold the liquid sample; a light source configured to emit coherent light to the sample cell; imaging optics configured to collect light scattered from the sample cell to a periphery thereof at different scattering angles, the imaging optics including a lens; a slit plate configured to limit a scattering angle range, the slit plate being arranged on an incident side of the imaging optics; a detector configured to receive collected light from the imaging optics; and an aperture plate configured to limit a width of light received by the detector by an aperture width, the aperture plate being arranged further toward a detector side than a focal length of the imaging optics, wherein a plurality of detection optical systems extending from the sample cell to the detector is arranged around the sample cell at equal intervals from a central axis of the sample cell, each of the plurality of detection optical systems having the imaging optics, the slit plate, the detector, and the aperture plate, the plurality of detection optical systems having a plurality of detectors, wherein the plurality of detectors includes at least three detectors, the at least three detectors including a first detector arranged at a first position closer to a reference position, a third detector arranged at a third position further away from the reference position, and a second detector arranged at a second position between the first detector and the third detector, the second position being closer to the reference position than the third position and further away from the reference position than the first position, the reference position being defined as a position at which an angle with respect to an incident direction of the coherent light to the sample cell is 90°, wherein the aperture width of the aperture for the first detector is larger than the aperture width of the aperture for the second detector and the aperture width of the aperture for the second detector is larger than the aperture width of the aperture for the third detector, and wherein the aperture width of each aperture plate is a value obtained by multiplying a distance from the central axis of the sample cell to the detector by a sine value of an arrangement angle of each detector. ### Claim 2 (depends on 1) The light-scattering detection device as recited in claim 1, wherein the aperture of the aperture plate is straight in at least a side extending along a vertical direction. ### Claim 3 (depends on 1) The light-scattering detection device as recited in claim 1, wherein the aperture plate is arranged rotatably in a horizontal direction to perform a fine adjustment of the aperture width. ### Claim 4 (depends on 3) The light-scattering detection device as recited in claim 3, wherein the aperture plate includes a controller for controlling a rotation angle of the aperture plate based on refractive index information of a solvent in the liquid sample. ### Claim 5 (depends on 4) The light-scattering detection device as recited in claim 4, wherein the aperture plate includes a rotating device for rotating the aperture plate and a storage for storing the refractive index information of the solvent in the liquid sample. ### Claim 6 (depends on 3) The light-scattering detection device as recited in claim 3, wherein the aperture plate is supported by a rotation shaft extending in a vertical direction, and the aperture plate is rotatable in the horizontal direction about the rotation shaft. ### Claim 7 (depends on 1) The light-scattering detection device as recited in claim 1, wherein each slit plate is formed to have a slit for limiting the scattering angle range, and wherein a width of each slit becomes maximum at an arrangement angle of 90° with respect to the incident direction of the coherent light to the sample cell and decreases as the arrangement angle deviates from the arrangement angle of 90°. ### Claim 8 (depends on 7) The light-scattering detection device as recited in claim 7, wherein the width of each slit is a value obtained by multiplying the width of the slit when the arrangement angle is 90° by a sine value of the arrangement angle of each detector. ### Claim 9 (depends on 1) The light-scattering detection device as recited in claim 1, wherein the light source is arranged such that an optical axis of the coherent light incident on the sample cell from the light source is inclined at a predetermined angle from a plane including the sample cell and the detector. ### Claim 10 (depends on 9) The light-scattering detection device as recited in claim 9, wherein the plane including the sample cell and the detector is a horizontal plane.