# US 12,181,398 B2 · Particle measuring device and particle measuring method

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- Number: US 12,181,398 B2
- Document ID: US12181398B2
- Status: Granted
- Kind: B2
- Application number: 17/558,289
- Filed: 2021-12-21
- Published: 2022-06-30
- Granted: 2024-12-31
- Earliest claimed: 2020-12-24
- Assignees: Rion Co., Ltd.
- Applicants: Rion Co., Ltd.
- Inventors: Mitsuaki Saito; Reiko Sugahara; Yuka Yoshikawa
- Web page: https://patentgrep.com/patent/US12181398B2

## CPC

- G01N 15/0205: by optical means
- G01N 15/1425: {using an analyser being characterised by its control arrangement}
- G01N 15/1429: Signal processing
- G01N 2015/0038: {Investigating nanoparticles}
- G01N 2015/1024: {Counting particles by non-optical means}
- G01N 2015/1486: {Counting the particles}
- G01N 2015/1493: {Particle size}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A particle measuring device includes: a detection unit that detects scattered light generated due to interaction between a particle contained in a liquid sample and light incident thereon, and converts the detected scattered light into a signal; an addition unit that performs a predetermined number of parallel processing on the signal to add the predetermined number of uncorrelated noises thereto and outputs the resulting signals; a binarization unit that binarizes the resulting signals using a binarization threshold set in accordance with the liquid sample, and outputs the binarized signals; a calculation unit that calculates and outputs a value based on the binarized signals; a filter unit that passes a predetermined frequency component of the output of the calculation unit; and a determination unit that determines that the particle is present when an output of the filter unit exceeds a predetermined particle threshold.

## Claims

### Claim 1

A particle measuring device comprising;
an optical detector configured to detect scattered light generated due to interaction between a particle contained in a liquid sample and light incident on the liquid sample, and convert the detected scattered light into a signal with a magnitude corresponding to intensity of the detected scattered light; and
a processor coupled to a memory storing instructions to permit the processor to function as:
an addition unit configured to perform a predetermined number of parallel processing on the signal to add a predetermined number of uncorrelated noises to the signal respectively and output resulting signals after the addition;
a binarization unit configured to binarize the resulting signals after the addition using a binarization threshold set in accordance with the liquid sample, and output the binarized signals;
a calculation unit configured to calculate and output a value based on the binarized signals;
a filter unit configured to pass a predetermined frequency component of the output of the calculation unit;
a determination unit configured to determine that the particle is present when an output of the filter unit exceeds a predetermined particle threshold determined in advance according to a minimum measurable particle size of the particle measuring device; and
a binarization threshold calculation unit configured to calculate the binarization threshold based on a characteristic amount of a scattered light component of the liquid sample.

### Claim 2 (depends on 1)

The particle measuring device according to claim 1,
wherein the binarization unit binarizes the resulting signals after the addition using the binarization threshold calculated by the binarization threshold calculation unit.

### Claim 3 (depends on 2)

The particle measuring device according to claim 2, wherein the binarization threshold calculation unit calculates the binarization threshold based on an RSM value or a DC value of the scattered light component of the liquid sample.

### Claim 4 (depends on 1)

The particle measuring device according to claim 1, further comprising:
a storage unit configured to store noise data having a predetermined data length,
wherein the processor further functions as a noise generation unit configured to generate the predetermined number of the noises as uncorrelated noises that are generated from the noise data using different starting points respectively, and
wherein the addition unit adds the predetermined number of the uncorrelated noises generated by the noise generation unit.

### Claim 5 (depends on 1)

The particle measuring device according to claim 1, wherein the calculation unit calculates and outputs an average value of the binarized signals.

### Claim 6

A particle measuring method comprising:
detecting scattered light generated due to interaction between a particle contained in a liquid sample and light incident on the liquid sample, and converting the detected scattered light into a signal with a magnitude corresponding to intensity of the detected scattered light;
performing a predetermined number of parallel processing on the signal to add a predetermined number of uncorrelated noises to the signal respectively and output resulting signals after the performing the predetermined number;
binarizing the resulting signals after the addition using a binarization threshold set in accordance with the liquid sample, and outputting the binarized signals;
calculating and outputting a value based on the binarized signals;
passing a predetermined frequency component of the output in the calculating and outputting the value;
determining that the particle is present when the output in the passing the predetermined frequency component exceeds a predetermined particle threshold determined in advance according to a minimum measurable particle size of a particle measuring device; and
calculating the binarization threshold based on a characteristic amount of a scattered light component of the liquid sample,
wherein, in the binarization, the resulting signals after the addition are binarized using the binarization threshold calculated by the binarization threshold calculation.

### Claim 7

A non-transitory computer readable medium encoded with a signal processing program for measuring a particle included in a liquid sample, that is to be executed by a computer including a processor coupled to a memory storing instructions to permit the processor to function as:
an addition unit that performs a predetermined number of parallel processing on a signal to add a predetermined number of uncorrelated noises to the signal respectively and outputs resulting signals after the addition, the signal having a magnitude corresponding to intensity of scattered light generated due to interaction between the particle and light incident on the liquid sample;
a binarization unit that binarizes the resulting signals after the addition using a binarization threshold set in accordance with the liquid sample, and outputs the binarized signals;
a calculation unit that calculates and outputs a value based on the binarized signals;
a filter unit that passes a predetermined frequency component of the output of the calculation unit;
a determination unit that determines that the particle is present when an output of the filter unit exceeds a predetermined particle threshold determined in advance according to a minimum measurable particle size of a particle measuring device; and
a binarization threshold calculation unit that calculates the binarization threshold based on a characteristic amount of a scattered light component of the liquid sample,
wherein the binarization unit binarizes the resulting signals after the addition using the binarization threshold calculated by the binarization threshold calculation unit.