Imaging system hardware

A method of assessing an imaging capability of an imaging system, the method comprising:

• identifying a frame comprising a plurality of metallic fiducial markers arranged on a surface of a substrate;
• illuminating the substrate using radiation at a first wavelength;
• measuring a light emitted by an array disposed on the surface within a perimeter defined by the frame, wherein the array comprises a plurality of non-metallic fluorescent markers, wherein the plurality of the non-metallic fluorescent markers includes a first non-metallic fluorescent marker comprising a first fluorescent probe;
• and
• determining whether the non-metallic fluorescent marker is present or absent on a visual representation of an object comprising relative dimensional information in at least two orthogonal spatial dimensions, wherein the visual representation is generated by the imaging system based on the light emitted by the array.