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US 12,180,541 B2Granted
Cell barcoding for single cell sequencing
AssigneeBio-Rad Laboratories, Inc.
InventorRonald Lebofsky
Life of this patent
Estimating the term…
Abstract
Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.
Claims11
A solid hydrogel around a cell mixture or nuclei mixture such that individual cells or individual cell nuclei are separated from each other by the hydrogel, wherein:
- the individual cells comprise heterologous oligonucleotides attached to cell membranes of the individual cells or the individual cell nuclei comprise heterologous oligonucleotides attached to nuclear membranes of the individual cell nuclei.
Dependencies are inferred from the claim text.