Composition for detecting dsRNAs, comprising merocyanine compound and isomer thereof, and method for providing information for diagnosing cancer, by using dsRNA expression analysis
AssigneeKorea Advanced Institute of Science and Technology
InventorsYoosik Kim, Raisa Kharbash, Ahsan Ausaf Ali, Minjeong Kang
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Abstract
The merocyanine compound of the present invention causes a spectral change of a hypochromic shift in the vicinity of the wavelength of 512 nm by intercalation between dsRNA nucleotide pairs, and the spectral change shows high accuracy and reproducibility, so the merocyanine compound of the present invention can be effectively used for detecting dsRNA and comparing the expression levels between samples. In addition, the present invention relates to a composition for detecting dsRNA comprising a merocyanine compound, a salt thereof or an isomer thereof.
Claims3
A method for detecting double-stranded RNA (dsRNA) comprising contacting in a sample containing the dsRNA after RNase T1 and DNase I treatment on total RNAs separated from each of control and experimental group samples with a composition comprising a merocyanine compound represented by formula 1 below, said method comprising:
- a) preparing a merocyanine compound represented by formula 1 below by irradiating a spiropyran compound represented by formula 2 below with ultraviolet light, wherein formula 1 is
- and formula 2
- and wherein in formula 1 and formula 2, R is —CH2CH2CH2N+(CH3)3
- b) preparing a control sample containing the dsRNA and the sample containing the dsRNA by treating total RNA extracted from a control group and total RNA extracted from a test subject with RNase T1 respectively;
- c) reacting the merocyanine compound of step a) with the control sample containing the dsRNA and the sample containing the dsRNA from step b) in a reaction solution;
- and
- d) calculating absorbance change rate at 512 nm using UV-Vis spectrometry by comparing absorbance in the control sample to absorbance in the sample containing the dsRNA from step b), wherein the absorbance change rate is proportion to dsRNA concentration.
Dependencies are inferred from the claim text.