Transformant, and method using said transformant to detect presence or absence of reduced phosphorous compound
AssigneeHiroshima University
InventorsRyuichi Hirota, Akio Kuroda, Kei Motomura
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AssigneeHiroshima University
InventorsRyuichi Hirota, Akio Kuroda, Kei Motomura
Estimating the term…
It is an object of an aspect of the present invention to provide (i) transformants, whose proliferation depends on phosphite, of various species of organism and (ii) a method for detecting the presence of a reduced phosphorous compound with use of such a transformant. Use is made of a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced, a signal peptide of a hypophosphite binding protein being substituted with a signal peptide derived from a host or a species of organism closely related to the host.
A cyanobacterial transformant comprising a disrupted gene encoding a phosphate transporter protein, a disrupted gene encoding a phosphate ester transporter protein, and an inserted gene encoding a hypophosphite transporter protein, wherein:
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